Abstract: Galactose-specific Pseudomonas aeruginosa lectin was successfully purified by affinity chromatography on D-galactose-starch gel, which was prepared by using cross-linking reagent epichlorohydrim, and the results involving its yield, molecular weight and amino acid composition were compared with those obtained by affinity chromatography on Sepharose 4B. The optimal conditions for purification with D-galactose-starch gel method were also observed.