The present study dealt with seve-ral influencing factors in quantita-tive ELISA technique for the detec -tion of IgM-RF and IgG-RF. We selected the appropriate conditions of the experiment and standarized them. we observed and analysed the interaction between IgM-RF and IgG-RF. The result showed that IgG--RF also influenced the detec-tion of IgM-RF, i. e. IgG--RF might elevate the level of IgM-RF. It was the first time we used the internatinal standarized product of RF of WHO in our country and standarized the RF serum which would be used as a control in our laboratory with the hope that it might lay a foundation for compa-ring the results among different laboratories here after. Clinically, we detected 367 cases of patients with five diseases (RA, SLE, HB, TB and GN) with this technique. The result showed that the signi-ficance of IgG-RF was not less than that of IgM-RF in some diseases. This technique is not only simple, stable and easy to be standarized, but also sensitive and specific. It can be applied in common laboratories and is of some help to appraise the curative effect and prognosis.
