Abstract:

The purpose of this work was to detct IK and CIC in the same serum by C_3b-ELISA. It was demonstrated that serum IK and CIC was separated effectively by an initial precipitation step using 3.5% PEG (M. W. 6000). IK contained in the supernatant and IC present in the solubilized precipitate were able to bind specifically to solid-phase C_3b. It was worthy of note that in sera of ICD patients with either positive IK or IC. the percentage of IK and IC double positive results varied in the tests from 45% to 96%. The kinetic observation on patients with IGD suggested that there existed a parallelism between the percentage of double postive and the severity of the disease. C_3b-ELISA is simple, rapid, and inexpensive to perform and it seems to provide a specific, sensitive, and versatile tool for the quantitative analysis of IK and IC.