Abstract: A new solid phase-dish coated with polystyren using sandwich ELISA to determine HBsAg was described in this paper. The results of dish-ELIS A and its sensitivity were compared with conventional polystyrene microtitration plate ELISA on 170 patients serum specimens. No significant difference was observed. This method was able to detect as little as 0.005 ug/ml of HBsAg. The advantages of dish-ELISA including sensitivity, simplicity, speediness and low cost render this solid phase suitable for ELISA.