Abstract:

Porcine Clq was purified by the euglobulin precipitation method of yonemasu and the affinity chromatography method of Bing. Contaminating IgG was absorbed away with lyophilyzed staph,aureus Cowan I type. Such Clq preparations were shown to contain IgM-RF by heat-inactivation studies and SDS-PAGE analysis. Highly purified porcine IgM had RF actively as well. When it was used in the solid phase Eliza test to detect CIC, the sensitivity range was 10-1000ng HAG/ml. 38 normal sera had values of 2.06±1.52 ng HAG/ml(m±SD). Sera from 13 cases of SLE and 16 cases of RA had positive rates of 31% and 62.5% respectively(2 SD). The satisfactory results performance and cheapness of the pig blood are in favour of the use of porcine Clq in CIC assays.