Abstract: The establishment of an immunoenzyme stain assay for screening mo-noclone antibody against chlamydia trachomatis is described. The results of the method in detecting monoclone antibody in culture supernants and ascitic fluids were basically the same as those by RIA and IFA. The method is simple, specific and rapid, and is suited for micro samples and particulate antigens. The prospects for using the method in diagnosis of eye diseases are discussed.

Key words: chlamydia trachomatis, micro sample, immunoenzyme