Abstract: Vertical electrophoresis was used in analyzing lactic dehydrogenase (LDH) isoenzymes in serum and subretinal fluid (SRF) of retinal detachment patients with and without high myopia. It was found that in retinal detachment, the serum LDH isoenzymes were essentially the same as those of the normal individual, both containing LDH₁, LDH₂, and LDH₃. But as the course of the disease was prolonged, the isoenzymes bacame increased both in their variety and in their content. In patients with detachments of over 6 months' duration, levels of LDH in the SRF were found to be higher than those in their own sera, indicating that LDH in the SRF came from retinal tissue as well as from the blood plasma.

Key words: lactic dehydrogenase isoenzymes, retinal detachment, sutrtienal fluid