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15 October 1988, Volume 9 Issue 4
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论著
The Investigation of the Pyramidal Tract in the Spinal Cord of the Rabbit
Gao Xiulai
1988, 9(4): 237-241.
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Anterograde degeneration of the pyramidal tract has been studied with the cupric silver method, the Fink-Heimer method procedure Ⅱ and bleaching of Fink-Hcimer method in 15 rabbits after lesions of frontal and parietal cortex. Degenerated fibers and terminals were observed under microscope and recorded by camera lucida. Experimental results showed that heavy degenerated fibers were found in the ipsilateral pyramid. Degenerated fibers crossed to the opposite side in the pyramidal decussation and lay within the gray matter of the dorsal horn in segments C
1
~C
5
. In segment C
1
heavy degenerated fibers mainly distributed in laminae Ⅲ~V of the spinal cord. Degenerated fibers gradually decreased and limited in lateral part of laminae V in segments C
2
~C
5
. Degenerated terminals were distinguished definitely in laminae Ⅲ~V throughout segments C
1
and C
2
. The resultssuggested that location of the pyramidal tract may reflect an aspect of evolution in mammals.
The Acetylcholinesterse Containing Neuros of the Spinocerebellar Tracts in the Cord of Rat by Using HRP-AChE Double Labeling Technique
Zhang Jinlu
1988, 9(4): 242-248.
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12 rats were used in present study for localization of the AChE-contianing neurons of the spinocerebellar tracts in the cord. 10 cases of them, the HRP-AChE double-labeled and HRP sing-labeled neurons in these gments of the spinal cord were traced with Mesulam's histochemical techrique. AChE-containing neurons and spinocerebellar neurons in the same segment of the spinal cord are demonstrated respectively in another 2 cases. The result of present study suggests that approximate 50 per cent of the spinocerbellar neurons in rat spinal cord contain AChE, although the amounts of AChE in those neurons may be different. The population of the AChE neurons in different cell-groups of the spinocerebellar tracts would be ralated with the functional differences between cell-groups.
A Histochemical Study on the Changes of the Endometrium During the Estrus Cycle and Early Pregnancy in Rats
Shi Xiaolin
1988, 9(4): 249-253.
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Histochemical changes of the superficial epithelium and the glandular epithelium during estrus cycle and early pregnancy in rats were investigated. The results indicated that during diestrus the activity of ACP, G6Pase, NSE was declined and the amounts of glycogen and lipid were quite low as well. During estrus the activity of NSE, ACP and SDH was increased, which suggested that the metabolism of carbohydrate and lipid became very active. At the 6th day of rat pregnancy the amounts of glycogen and lipid and the activity of ACP, G6Pase, NSE were clearly raised, which suggested that the nutrition of blastocyst during implantation probably provided by the superficial epithelium. In addition, the superficial epithelium showed positive reaction to 3-β-HSDH, but its significance remains to be studied.
The Differentiation Ectopic Pregnancy and Normal Pregnancy by Immunohistochemical Assay
Zhu Dingshou;L. Moyer
1988, 9(4): 254-257.
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Immunohistochemical demonstration of placental hormones and pregnancy specific β-1-glycoprotein of villus is a usful tool in the differentiation of normal pregnancy and ectopic pregnancy. There were 57 samples in the study. We did not find chorionic villus in all endometrium curetting or spontaneously passed tissue. Using specific antiserum we detected human chorionic gonadotropin (HCG), (human) placental lactogen (HPL) and pregnancy specific β-1-glycoprotein (PSI) in the trophoblast of villus. The 18 of normal pregnancy specimens were marked with HCG, HPL and PSI. The other 37 specimens which were ectopic pregnancy were not marked. Therefore, we concluded that the use of these hormones marks in endometrium specimens significatly increased the precision of the diagnosis for the ectopic pregnancy.
A Comparative Study on Effect of α-Mercapto-β-(2-Furan)-Acrylic Acid on the Combination of Human Serum Albumin with Nickel and Zinc
Zhang Yingxia
1988, 9(4): 258-262.
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Having prepared α-mercapto-β-(2-furan)-acrylic acid in the laboratory, this work makes a study on effect of α-mercapto-β-(2-furan)-acrylic acid on combination of Human Serum Albumin with Zine, Nickel respectively by gel chromatography. The results showed that the combination of Nickel with Human Serum Albumin, likes Zine, is remarkably strengthened under existence of α-mercapto-β-(2-furan)- acrylic acid, which indicates that mechanism of detoxication of Nickel can not be explained oniy by competition among them small ligands.
The Effects of the Fine Extract of Poacynum Pictum Baill on Drugemetabolizing Enzymes of the Liver in Rats
Xing Weirong
1988, 9(4): 263-265.
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In this paper the influences of the fine extract of poacynum pictum baill on drug-metabolizing enzymes of the rat liver were studied. The results indicated that the fine extract of poacynum pictum baill decreased the content of cytochrome P
450
and the activity of NADPH- cytochrome creductase of the rat liver, but uneffected the activity of heptic GSH S-transferase.
Lactic Dehydrogenase Isoenzyme in the Subretinal Fluid of Patient with Retinal Detachment
Meng Xianmin;Wang Jingzhao
1988, 9(4): 266-268.
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Vertical electrophoresis was used in analyzing lactic dehydrogenase (LDH) isoenzymes in serum and subretinal fluid (SRF) of retinal detachment patients with and without high myopia. It was found that in retinal detachment, the serum LDH isoenzymes were essentially the same as those of the normal individual, both containing LDH
1
, LDH
2
, and LDH
3
. But as the course of the disease was prolonged, the isoenzymes bacame increased both in their variety and in their content. In patients with detachments of over 6 months' duration, levels of LDH in the SRF were found to be higher than those in their own sera, indicating that LDH in the SRF came from retinal tissue as well as from the blood plasma.
A Thymie Morphologic Study of 285 Autopsies in Fetus and Infants
Tang Xiaonai
1988, 9(4): 269-273.
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The article presents a study of the weight and histomorphological changes of thymus in 285 fetus and infants. Among them 121 cases were normal thymus, 156 cases were involution and 8 cases were underdevelopment. The average weight of normal thymus in newborn infants was about 16 g. Involution frequently appeared in pneumonia. The more severe involution was, the longer duration was. The factors in relationship with thymic weight、causes of involution and congenital immunodeficiency were discussed.
Massive Pulmonary Embolism
Xiong Zhiquan;Tang Xiaonai
1988, 9(4): 274-277.
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Massive pulmonary embolism is a embolic embolism and thrombosis in the pulmonary trunk and the left or right pulmonary arterier. In the 5 cases of massive pulmonary embolism, 4 of them were pulmonary thrombosis associated with chronic pulmonary heart disease and the pulmonary hypertension and andere case of pulmonary embolism occurred in a patient with kidney carcinoma. Sudden death appeared in 5 cases. None of the patients were correctly diagnosed before death as to the massive pulmonary embolism. In the this paper the incidence, pathogenesis, pathologic changes and clinical manifestation of massive pulmonary embolism were discussed.
Measurement of Testosterone of Plasma by RIA
Cao Junqing
1988, 9(4): 278-282.
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This article introduces the method for the determinatiom of testosterone in plasma by RIA. The antigen was prepared by compling testosterone with BSA. Antigen was used for preparing the rabbit antiserum. The separation of immunocomplex from the free form of testosterone was accomplished by 1%Charcoal. The sensitivity of this method is 0.07 pmol. The interassay and intraassay coefficients of variation were 4.7% and 16.4% respectively The average recovery rate of testosterone was 100.4%. The article reported the plasma testosterone concentration of 158 normal subjects measured by this method. The male plasma testosterone level of the group old than 60 is lower than the group under 60, The female plasma testosterone level during the climacteric and menopause is lower than child bearing period and there is not statistic different in the different period of menstruation circle.
A Comparitive Study between the Radiation Appearance and Pathology on 23 Cases of Fibro-tumours the Maxillo-facial Bone
Yang Dabai;Zhang Mao
1988, 9(4): 283-285.
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23 cases of fibrous tumours in the maxillo-facial bone were comparati vely studied between their radiation appearanecs and pathology. Among of cases, 12 cases were ossifying fibroma, 7 cases were cementifying fibroma, 3 cases were odontogenic fibroma. The relationship between the pathological structures of every kind of fibro-tumours and X-ray density were comparatively observed. The result indicated that although the initial diagnosis for ossifying-fibroma might be aquired according to the X-ray findings, the ultimate diagnosis should be obtained combined with the pathological structures. Cementifying fibroma might be diagnosed by X-ray findings alone.
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