Journal of Capital Medical University ›› 1989, Vol. 10 ›› Issue (1): 15-18.

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The Screening of cDNA Clones of Human Serum Albumin Using Enzyme Labeled Immuno Assay and Their Identification

Zhang Chengbo1   

  1. 1. Department of Cell Biology, Capital Institute of Medicine
  • Received:1988-10-06 Revised:1900-01-01 Online:1989-01-15 Published:1989-01-15

Abstract: Using enzyme labeled immuno assay, the procedures of screening the clones of albumin cDNA from a library of human hepatic cells were reported. These selected clones were identified with immunological method and res- triction enzymes digestion, of the seven positive clones, the sum of three overlaps the whole length of coding region of albumin.

Key words: human serum albumin, cDNA clone, enzyme labeled immuno assay, E. Coli.