Journal of Capital Medical University ›› 1989, Vol. 10 ›› Issue (4): 252-255.

• 论著 • Previous Articles     Next Articles

Molecular Cloning of Human Papillomavirus Type 11 DNA

Sun Wensheng1, Cao Yinglin1, Li Hui1, Zhang Lining2, Gao Tianxiang2   

  1. 1. Laboratory of Electron Microscope, Shandong Medical Institute;2. Laboratory of Electron Microscope, Capital Institute of Medicine
  • Received:1989-02-24 Revised:1900-01-01 Online:1989-10-15 Published:1989-10-15

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Abstract: Successful transformation have been mede by using the recombinant plasmid of human papillomavirus type11 DNA(HPV11DNA) and pBR322 plasmid vector with E coli C600 as the receptor bacteria. Sixty-one strains were obtained and fifteen strains were selected randomly for further identification. The pBR322-HPV11DNA plasmid were isolated by both the alkaline denature method and the clear cleavage method and cut by BamHI. Then the agarose gel electrophoresis has been done. The molecular weight of HPV11DNA was assayed by compairing with the reference DNA of standard bacteriophage which was cleaved with HindⅢ. The results showed there was the DNA band of HPV11 DNA with the MW of approximately 5.5×106dts and 8.1kb. So it is convenient for the further amplification of the pure HPV11DNA and production of the genetic probe for exploring the association of the HPV11 infection with the development of sequamous cancers.

Key words: pBR322-HPV11 DNA, transformation, DNA cloning