Journal of Capital Medical University ›› 1989, Vol. 10 ›› Issue (4): 256-259.

• 论著 • Previous Articles     Next Articles

Detection of Viral Sequences in DNA Extracted from Pathological Tissues of Carcinoma Cervix by Using 32P-Labelled Human Papillomavirus DNA Probes

Liu Xiaoming1, Gao Tianxiang2, Zhao Lixin3   

  1. 1. Laboratory of Electron Microscope, Shandong Medical University;2. Laboratory of Electron Microscope, Capital Institute of Medicine;3. Gynaecology and Obstetrics, Shandong People's Hospital
  • Received:1989-02-15 Revised:1900-01-01 Online:1989-10-15 Published:1989-10-15

PDF

200

Abstract: Southern blot analysis of human papillomavirus genome was carried out on 33 cases of human cervical specimens histologically diagnosed as cervical cacinoma (26 cases), condyloma acuminatum (1 case), vulvar carcinoma (1 case) and chronic inflammation (4 cases). 69% of cervical carcinoma were found to be positive for HPV DNA. Among them 23% (6/26) were positive for HPV 16,7.6% (2/26) were positive for HPV 18 and 11.5% (3/26) were positive for HPV X1.

Key words: southern blot hybridization, human papillomaviruses(HPV), cervical carcinoma