Abstract: For enhancing the purification recovery of carboxypeptidase B, the Felk’ s method was modified as following: (1)Both of Tris-HCl buffers with contained 0.1 mol/L and 0.15 mol/L NaCl were ap-plied respectively in the batch adsorption and elution from DEAE-cellulose.(2)In the second DEAE-cellulose chromatography, the DEAE-cellulose column was e-luted with linear gradient of NaCl from 0.00 to 0.09 mol/L in Tris-HAc buffer.(3)Carboxypeptidase B was eluted completely with 0.09 mol/L NaCl in Tris-HAc buffer.The purity and the molecular weight were identified by TLC scanning method after gel electrophoresis.The results showed that recovery of enzyme activity was in creased from 8% to 15%, specific activity was increased from 175 U/mg to 192 U/mg and purity of en-zyme was reached 91%.

Key words: carboxypeptidase, porcine pancreas, separation, punification