Journal of Capital Medical University ›› 1993, Vol. 14 ›› Issue (4): 280-283.

• 论著 • Previous Articles     Next Articles

A Study of ENA Antibodies with Counterimmuno-electrophoresis(CIE),One and Two Dimensional Electrophoresis and Immunoblotting(1D-IB,2D-IB)

Yu Naichang, Wang Hong, Sun Ying, Gao Changqing   

  1. Xuanwu Hospital, Capital Institute of Medicine
  • Received:1992-07-05 Revised:1900-01-01 Online:1993-10-15 Published:1993-10-15

PDF

258

Abstract: Twenty SLE sera were examined for ENA autoantibodies with calf thymus and human spleen extracts,and HEP-2 cell lysate as antigens in CIE,and HEP-2 lysate as antigen in 1D-IB and 2D-IB.1D-IB was more sensitive than CIE for the detection of Sm antibody only. 2D-IB could effectively detect SS-A and SS-B antibodies and analyze their fine specificities; it could demonstrate RNP antibodies reacting with 68 kD and 20 kD polypeptides,but had a low detection rate for Sm antibody(3 positives out of 20 sera).The use of HEP-2 cell lysate as antigen in CIE yielded a low detection rate for SS-B antibody(2/20).

Key words: electropholysis, connective tissue disease, systemic lupus erythematosus

CLC Number: