Journal of Capital Medical University ›› 1994, Vol. 15 ›› Issue (4): 270-274.

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The Study of Solvent Efficiency in Ganglioside Preparation

Pan Ying, Huang Rubin, Wang Zesheng, Tong Dashan, Shi Xiaoling   

  1. Department of Biochemistry, Capital Institute of Medicine
  • Received:1993-12-09 Revised:1900-01-01 Online:1994-10-15 Published:1994-10-15

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Abstract:

Gangliosides(Gls)content is very low in organism. The complex procedures of isolation and purification of Gls expended large amount of solvent. The technique of the centrifugal liquid chromatography and recovery of waste solvent were effective steps improved solvent efficiency in Gls preparation. The consumption of solvent went down by 88. 5% from 17.5L.The productivity of pig brain Gls was 0.0894%.

Key words: gangliosides(Gls), solvent, centrifugal liquid chromatography(CLC)

CLC Number: