Abstract:

Refrigerated follicular cells that were taken from ovaries of embryonal, adult and pregnant rats were resuscitated and cultured in RPMI 640. The morphological study indicated that all the resuscitated cells had the proliferative capability in culture medium. But the embryonal follicular cells lost the proliferative capability from the 4th day in culture. The cytochemical study showed that embryonal follicular cells were negative to 3-β-HSDH from the 3rd day. The study demonstrated that embryonal follicular cells are immature cells, but they have potential endocrine function.

Key words: follicle cell, cell culture, refrigeration