Table of Content

15 July 1998, Volume 19 Issue 3

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A Study of Follicular Cells of Adult and Embryonal Rats in Vitro

Shi Xiaolin;Xu Qing;Li Weihong;Zhu Dingshou

1998, 19(3):  201-204. 

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Refrigerated follicular cells that were taken from ovaries of embryonal, adult and pregnant rats were resuscitated and cultured in RPMI 640. The morphological study indicated that all the resuscitated cells had the proliferative capability in culture medium. But the embryonal follicular cells lost the proliferative capability from the 4th day in culture. The cytochemical study showed that embryonal follicular cells were negative to 3-β-HSDH from the 3rd day. The study demonstrated that embryonal follicular cells are immature cells, but they have potential endocrine function.

Antisense c-myc Oligodeoxynucleotides Inhibited Proliferation, Migration of Vascular Smooth Muscle Cells and Induced Apoptosis by Downregulation of c-myc Expression

Ma Yaluan;Gao Tianxiang;Wang Jianing;Qi Yahui;Yin Hong;Feng Guifen

1998, 19(3):  205-208. 

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To investigate the effects of synthetic phosphorothioate-modified antisense oligodeoxynucleotides(ODN), which is complementary to the first five codens of c-myc mRNA(AS ODN), on proliferation, migration and apoptosis of vascular smooth muscle cells(SMC), AS ODN were introduced into cultured rat arotic SMCs by lipofectin, observed that antisense ODN inhibited SMC proliferation and migration, and induced SMC apoptosis. These effects were associated with decreased expression of c-myc encoded protein which was shown by western blot. ODN with the same nucleotides but a sense sequence or a mismatched sequence did not show same effects as AS ODN did. The results suggested a potential role of antisense strategies designed to inhibit c-myc expression for prevention of restenosis following angioplasty.

论著

A Comparative Study on Ovary Autotransplantation and Fetal Ovary Allotransplantation

Liang Yuanjing;Zhu Dingshou;Shi Xiaolin;Weng Jing;Lu Xin;Xu Qing

1998, 19(3):  209-212. 

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Ovary autotransplantation and fetal ovary allotransplantation were performed in female mice. Estrus cycle was observed before and after transplantation. Histological study and radioimmunoassay were used to study the graft growth, and the proper time for fetal transplantation. 14 d after ovary autotransplantation, the ovarian structure showed normal. Serum P level increased. The estrus cycle reoccured. 7 to 14 d after fetal ovary(17th day gestation)allotransplantation, primordial follicles and preantral follicles could be found. This study indicates that fetal ovary(17th day of gestation)can grow and become mature after allotransplantation.

Effect of Monosodium Glutamate on GTH Pituicyte in Rats

Liu Dongmei;Yang Peisun;Wang Pengwen;Zhou Yijian

1998, 19(3):  213-216. 

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The neonatal rats were injected subcutaneously with 10% monosodium glutamate(MSG). The development of rat pituitary gland and LH pituicyte were observed on the 52th day(puberty)and 100th day(adult). The results showed:The pituitary weights on 52th and 100th day were(3.26± 0.51)mg and(5.21±0.72)mg in MSG group respectively. They decreased significantly as compared with control group(P<0.01),(8.46±0.81)mg and(12.04±1.23)mg respectively. The mean area of LH pituicytes on 52th day and 100th day were 998.00±161.56 and 1 105.56± 219.83. They decreased remarkably as compared with control group(P<0.01), 1 662.30±141.46 and 1 892.11±212.29 respectively. However, LH content of individual LH pituicyte in MSG group had no significant differences as compared with that in control group(P>0.05). The serum concentrations of LH and FSH were significantly lower in MSG group than those of control group on both ages. A striking reduction in the number of neurons in the arcuate nucleus(ARC)were noted. Thus, MSG can depress the development and function of GTH pituicytes.

Protective Effects of Electro-Acupuncture on Focal Cerebral Ischemia Reperfusion Injury in Rats

Zhang Xueqin;Zuo Dapeng;Wang Qiuping;Zhao Yi;Gao Yuhong;Geng Zhiping;Chen Zhiqiang

1998, 19(3):  217-220. 

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Using a rat model of reversible regional cerebral ischemia, the effects of electro-acupuncture were examined at the point of “huantiao” before reperfusion. The left middle cerebral artery(MCA)occlusion was achieved with a nylon filament placed into the vessel. The results showed that animals in the acupuncture treatment group had a significantly regaining of movement ability than that in the ischemia reperfusion group. The mean levels of malondialdehyde(MDA)concentration and superoxide dismutase(SOD)activity of the ischemic hemisphere in the acupuncture treatment group returned to the level of sham control group. The cortical somatosensory evoked potentials(SEP)measured ipsilaterally on the cortex of ischemic hemisphere showed that with electro acupuncture,both P1 latency and P1-N1 amplitude improved significantly. The results suggested that electro-acupuncturing at the point of “huantiao” has beneficial effects on focal cerebral ischemia-reperfusion injury in rats.

Correlation between Systolic and Diastolic Blood Pressure and Left Ventricular Systolic and Diastolic Function in Patients with Essential Hypertension

Hua Qi;Wang Xifeng;Xing Hua;Li Xiuzhen;Xing Jiangang

1998, 19(3):  221-224. 

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To investigate the correlation between systolic and diastolic blood pressure and systolic and diastolic function of left ventricle, blood pressure and left ventricular function were observed by echocardiography in 374 patients with essential hypertension. It was found that there were negative correlation between systolic blood pressure(SBP)and peak flow velocity of early diastole(EPFV)(P<0.05), positive correlation between SBP and peak flow velocity of atrial contraction(APFV)(P<0.05)and between SBP and A/E(P<0.01); and there was negative correlation between diastolic blood pressure(DBP)and cardiac output(CO)(P<0.01). Cholesterol was higher in patients with isolated systolic hypertension than patients with diastolic hypertension(P<0.001). Stepwise multivariate regression analysis disclosed that A/E ratio all alone and jointly positively correlated with SBP, and age and CO all alone and jointly correlated with DBP. It was suggested that left ventricular diastolic function was mainly influenced by SBP and systolic function was mainly influenced by DBP.

The Delayed Antiarrhythmic Effects of Femoral Artery Ischemic Preconditioning in Rats

He Feng;Wang Jiarui;Lu Lingqiao;Zhu Yingfen;Zhang Like

1998, 19(3):  225-227. 

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The delayed antiarrythmic effects of femoral artery ischemic preconditioning(FIP)in rats were observed. Results: During the cardiac ischemia, the incidences of VT,VF were significantly reduced and the accumulative total periods of VT,VF were significantly shortened in group FIP compared with in group NIP(P<0.01). The incidences of reperfusion induced VT,VF and the mortality in group FIP were not significantly reduced from NIP group(P=NS). But in group FIP 24 h, the incidences of reperfusion induced VT,VF were significantly reduced and the mortality was significantly reduced from NIP group of 50% to 0%(P<0.05). The results suggest that the protective effects of FIP against ischemia and reperfusion induced arrhythmias in rats are obvious. The delayed antiarrythmic effects of FIP are more effective than the early ones and reducing the incidence of reperfusion induced IVF and increasing the survival are their dominant effects.

The Relationship between Insertion/deletion Polymorphism of Angiotension-converting Enzyme Gene and Coronary Heart Disease

Gu Haitong;Zhang Husheng;Wang Yong;Jiang Yanfang

1998, 19(3):  228-230. 

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Insertion/deletion(I/D)polymorphism of the ACE gene was detected by polymerase chain reaction(PCR)in a study of 100 control subject and 95 patients with coronary heart disease(CHD), and was campared with serum ACE level and CHD morbidity in Beijing population and prodisposing factors. Results: The frenquencies of D allele(0.54)and DD genotype(0.35)were higher among the CHD group than among the control subjects(0.35,0.13,both P<0.01). Conclution: Deletion polymorphism of the ACE gene might be an important risk factor for CHD in Chinese population.

Assessment of Myocardial Viability of Patients with Myocardial Infarction by ^99mTc-MIBI SPECT after Intravenous NTG Infusion

Yang Honglian;Du Fenghe;Wang Jie

1998, 19(3):  231-233. 

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To compare the clinical effect rest ^99mTc-MIBI myocardial tomography with that folling intravenous infusion of nitroglycerin(NTG)in assessing myocardial viability. Rest^99mTc-MIBI myocardial tomograthy imagings were performed in 57 patients with myocardial infarction. There were 207(40.4%, of 513)segments of hypoperfusion in the rest imagings of the 57 patients. The improvements of perfusion after NTG infusions were revealed to be in 75(36.2%)of the 207 segments. Combination of rest ^99mTc-MIBI myocardial tomography imaging with intravenous NTG infusion could efficiently detect the residual survival myocardium in the patient with myocardial infarction.

Homozygous Deletion of p16 Gene in Leukemia

Tan Shuzhen;Chen Wenming;Zhu Jiazhi;Liu Jingzhong

1998, 19(3):  234-236. 

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To investigate the incidence of p16 gene homozygous deletion in leukemia, and the relations between p16 gene homozygous deletion and the prognosis of leukemia, 48 patients were studied by multiplex polymerase chain reaction(PCR)to detect p16 gene homozygous deletion. P16 gene homozygous deletion were detected in 4 of the 48 patients. They were two patients with M₂, one patient with NHL accompany with ALL, and one patient with CML in blast crisis, respectively. P16 gene homozygous deletion correlates with adverse prognosis features. The patients with p16 homozygous deletion had poor response to chemotherapy, and died shortly after p16 gene homozygous deletion was detected. The homozygous deletion of p16 gene palys an improtent role in the pathogenesis and the development of some leukemia. The patients with p16 gene homozygous deletion have a poor prognosis.

Amp-FLP Analysis on D5S436 Locus and Its Application to Linkage Analysis in Gene Diagnosis of Asthma

Huang Kewu;Weng Xinzhi;Liu Jingzhong;Zhu Zhangling;Xiao Bai;Yan Mei;Zhang Hongyu;Wang Chen

1998, 19(3):  237-240. 

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To detect the polymorphsism of D5S436 locus by using amplified fragment length polymorphism(Amp-FLP), allele and genotype frequencies of the hightly polymorphic D5S436 locus were determined in 92 unrelated Chinese individuals. The amplified fragments were separated by denaturing polyacrylamide gel electrophoresis and the gel was silver stained. 8 alleles and 22 genotypes were observed in this population. The heterozygosity was 78.26%. The polymorphism information content(PIC)is 0.79. Amp-FLP genotyping linkage analyses were carried out in four asthma families, demonstrated that the locus was inherited according to Mendelian law and have a clear result. It is concluded that D5S436 can be used as a genetic marker of asthma in Chinese and maybe helpful in the gene diagnosis of asthma.

The Effect of Prevention and Treatment of Bisoprolol on Ventricular Arrhythmia Caused by Intracerebral Hemorrhage

Zhang Tong;Du Fenghe;Li Mingyue

1998, 19(3):  241-242. 

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Dynamic electrocardiogram and intracerebral hemorrhage model was used,to study the effect of prevention and treatment of bisoprolol on arrhythmia after brain injury in rats. The result shows that bisoprolol may reduce the occurrence of premature ventricular beat especially ventricular tachycardia.

Mearurement of Postmortem Extravascular Lung Water by Hemoglobin and Comparison to Parameter of Pulmonery Edema

Yan Tiansheng;Wen Guangnan;Jia Bin

1998, 19(3):  243-245. 

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To measure postmortem extravascular lung water(ELW)by using the non-isotope method and to compare to the various parameters of pulmonary edema, 21 canines were divided into two groups, control and pulmonary edema. After canines were put to death, the animal lungs were homogenated, weighted and roasted. ELW were measured by adapting the hemoglobin method. Multinomial parameters of the pulmonary edema were calculated by standard formulae. In control group ELW, extravascular dry weight(EDW)and lung total water(LTW)were(4.46±0.30)mL/kg,(1.48±0.20)mL/kg and(7.56±0.98)mL/kg, respectively. The results showed that the method of measuring postmortem, ELW was simplified and the measuring errors was reduced. Among the various parameters of pulmonary edema ELW was the most sensitive in responding to the change of the lung water. EDW can be used to show the type of pulmonary edema.

Influcence of KCl on Release of Glutamic ACID from Neuroblastoma Cell Line

Lu Xiaoli;Gao Qingling;Ding Yuji Chen Wendong;Li Yali;Li Lin

1998, 19(3):  246-249. 

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The influence of KCl on the release of glutamic acid from culture human neuroblastoma cell line(SH-SY5Y)was observed by using HPLC. The results of the experiments showed that KCl(10～30 mmol/L)incresed the concentration of glutamic acid in the cell culture when calcium ion existed in the culture medium. When the concentration of KCl above 40 mmol/L, there was no increment of glutamic acid released from the cell. The increase of glutamic acid induced by KCl was not found when there was no calcium ion in the culture medium. The results indicate that calcium ion is an important facto in the release of glutamic acid induced by KCl in SH-SY5Y cell culture.

The Value of Computeriged Tomography in the Investigation of Unexplained Hemoptysis

Zhang Jian;Cao Baoxin

1998, 19(3):  250-252. 

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The purpose of this study is to evaluate the use of CT in the investigation of unexplained hemoptysis. Those patients who were diagnosed normal through chest radiographs and examinations of the sputum forcells and tubercle bacillus were studied by CT from lung apex to diaphragm. Abnormalities were seen in 26(65%)of the CT scans. Fifteen of the patients had evidence of bronchiectasis(37.5%). In four cases(10%)infiltrative pulmonary tuberculosis was detected. In four cases(10%)pneumonia was read and lung cysts were shown in the final three(7.5%). The results showed that CT scan can be used as a routine study for suspected hemoptysis and most of the unexplained hemoptysis cases are bronchiectasis, pulmonary tuberculosis and pneumonia.

Effect of Rapid Induction of Anesthesia with Inhalation of Sevoflurane before/after Intravenous Infusion of Low dose Fentanyl/droperidol

Zhao Zhidan;Pang Jie;Li Shuren

1998, 19(3):  253-255. 

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To investigate the effect of rapid induction of anesthesia with inhalation of sevoflurane before/after intravenous infusion of low-dose fentanyl/droperidol.Sixteen ASA Ⅰ～Ⅱ patients under general anesthesia were randomly divided into two groups. In group 1: the 2 μg/kg fentanyl and 0.1 mg/kg droperidol were administrated intravenously during the 5 min after inhalation of sevoflurane, and in group 2: the 2 μg/kg fentanyl and 0.1 mg/kg droperidol were administered intravenously during the 2 min before induction.The results showed that the time necessary for loss of consciousness in group 1 and group 2 was(87±37)s,(39±11)s, and vital capacity breath frequency in group 1 and group 2 was 14.0±5.3, 4.0±0.7 respectively. Changes in blood pressure and heart rate were relatively small. The study shows that induction of anesthesia with inhalation of sevoflurane after intravenous infusion low dose fentanyl droperidol was found to be acceptable.

A Biomechanical Study of Discectomy on the Sagittal Stability of Lumber Spine

Li Qiang;Yang Yong;Zhao Yi

1998, 19(3):  256-259. 

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In order to improve the outcome of lumber discectomy, a biomechanical study was performed to identify the effects of posterior approach of lumber operation on the sagittal stability of lumber spine. Comparing with the displacements of normal disc group, the horizontal displacements of disc were slightly increased in the group that underwent decompression on the posterior outer annulus fibrosus(P>0.05), and significant increased in the group that underwent entire nucleus pulposus removal and the group that underwent curettage of cartilage end plate(P<0.01). The results suggested that single level discectomy of lumber spine trends to occur sagittal segmental instability after surgery. This should be given more consideration on the clinical setting.There is no relationship between the extent of sagittal segmental instability and the content of disc removal under low physiological shearing force. When the force gets higher, the outcome goes reversal.

Clinical Significance of Variation of Serum Lipids Levels and Marker of Thrombin Activation in Patients with Diabetics

Zhang Kuimei;Yang Yi;He Shida

1998, 19(3):  260-261. 

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For investigating the variation of lipids and thrombin activation in patients with diabetes, metabolism of lipids and marker of thrombin activation in 20 patients with diabetes and 20 normal subjects were observed. It was found that Ox-LDL, apoA and Lp(a)were significantly higher and PGA were significantly lower in diabetic group than in normal group, there were negative correlation between GHb and PAI. It was suggested that there were significantly abnormal metabolism of lipid and thrombin activation in patients with diabetes and diabetes is one of the important dangerous factor inducing atherosclerosis.

Dynamic Traction for Postoperative Management of Flexor Tendon Repair

Lin Yuan;Liu Chongjing

1998, 19(3):  262-263. 

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Using dynamic traction as a postoperative management to rehabilitate flexor tendon function(experimental group 25 cases).The experimental group(EG)are instructed keeping the hand in a protective position and allowing the fingers to do exercise of active extension and passive flexion at the first day of the postoperation.Results: The rate of rehabilitating the reasonable hand function. Experimental group: 92%,comparison group(CG)8%(P<0.01). The rate of flexor tendon adhesion,EG 8%,CG 75%(P<0.01). Results suggest: It is curative effect and easy to application in clinical works.