Journal of Capital Medical University ›› 1999, Vol. 20 ›› Issue (2): 83-85.
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Li Aihua1, Liu Tiancheng2, Tian Jingsheng2
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Abstract: The fulllength HSV1-tk gene was inserted into retrovirus vector pN2Aby DNA recombinant techniques. The plasmid pN2A-HSV1-tk was transfected into ψ2, PA317 packaging cell line by DNA-calcium phosphate coprecipitation. The G418 resistant clones were selected and their medium could infect NIH3T3 successfully and their average titer was 6.2×105CFU/mL. The highest one reached 1.5×106CFU/mL. The producer cell line will be used in gene therapy.
Key words: HSV1-tk gene, retrovirus vector, gene transfer
CLC Number:
Q784
Li Aihua;Liu Tiancheng;Tian Jingsheng. The Construction of pN2A-HSV1-tk and Expression[J]. Journal of Capital Medical University, 1999, 20(2): 83-85.
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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/
https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y1999/V20/I2/83
