Abstract: To clone the gene of human glial cell line-derived neurotropic factor for researching its effects in the nervous system diseases PCR primers were synthesized based on the hGDNF cDNA sequence, RT PCR and PCR were performed respectively on RNA and genomic DNA extracted from fetal brain.The PCR frag ment was subcloned into pGEM-T easy vector and then was confirmed by DNA sequencing.Results : The length of the two DNA fragments amplified by RNA and genomic DNA was both 410 bp.There was no difference between them.Restriction digests were analyzed to determine insert orientation.the fragment DNA sequences were homology with corresponding sequences published in Genebank.Conclusion :The hGDNF fragment has nointron because RT PCR and PCR am plifications are the same size ,thisfragment can be used in eukaryotic and prokaryotic gene expression.

Key words: human GDNF, neurotropic factor, gene clone