Journal of Capital Medical University ›› 2001, Vol. 22 ›› Issue (1): 24-26.

• 论著 • Previous Articles     Next Articles

The Preparation of Replication-deficient Recombinant Adenovirus AdE1CMV-NGF

Liu Shiquan1, Zhang Wei2, Jia Donghui3, Wang Shenwu1, Zheng Shuying1, Wang Wei4, Zhang Huirong4   

  1. 1. Center Laboratory, People's Hospital, Beijing Medical University;2. Beijing Friendship Hospital;3. Chinese Academy of Medical Science;4. The Third Clinical College, Beijing Medical University
  • Received:2000-01-29 Revised:1900-01-01 Online:2001-01-15 Published:2001-01-15

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Abstract: With the DNA recombinant techniques, we constructed a secretable form of adenoviral shuttle plasmid pAdE1CMV-NGF through sub-cloning and cloning steps. And then pAdE1CMV-NGF was cotransfected with pJM17 in 293 cells with the application of FuGENE 6 to produce the replication deficient virions containing the cDNA of interest, AdE1CMV-NGF. The titer reached 4.6×1010 PFU/mL by grade-dilution evaluation.

Key words: NGF gene, adenovirus vector, gene transfer, homologous recombination

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