Abstract:

To study the protective action of cerebral homogenate extracts on in vitro preparation, the present experiment was conducted on a hypoxic model of PC12 cells. LDH and MTT assay were used to measure changes of the cells′activity following incubation with brain tissue extracts taken from hypoxia preconditioned mice for 24, 48 and 72 h. The cells′activity was found to be significantly increased when the cells were incubated with the extracts containing protein and no significant change was seen when the cells were incubated with protein free extracts. The result indicates that protein like neuroprotective substance(s)is developed in the brain of hypoxia preconditioned mice.

Key words: preconditioning, hypoxia, PC12 cells, MTT assay