Journal of Capital Medical University ›› 2001, Vol. 22 ›› Issue (4): 300-303.

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Study on Cloning Human Endostatin Gene

Liu Hua, Zhang Chengbo, Xiong Ying, Chen Rui, Yu Peilan   

  1. Department of Biochemistry, Capital Uuiversity of Medical Sciences
  • Received:2001-07-12 Revised:1900-01-01 Online:2001-10-15 Published:2001-10-15

Abstract: In order to obtain the human endostatin gene, the total RNA was isolated from human fetus liver tissue, and two pairs of specific primers were designed for RT-CR. The target DNA fragment was inserted into the clone vector pBluescript and the expression vector pGEX-4T-1 respectively. The recombinant plasmids were used for sequencing.The result showed that the DNA sequence of inserted fragment was consistent with human endostatin mRNA in GenBank database.

Key words: endostatin, RT-PCR, clone

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