Journal of Capital Medical University ›› 2002, Vol. 23 ›› Issue (2): 101-104.

• 基础研究 • Previous Articles     Next Articles

Preliminary Purification and Pursuit of Evolutionary Origin of P-hydroxybenzoate 3-hydroxylase from Comamonas Testosteroni

Chen Rui1, Hosokawa Keiichi2   

  1. 1. Department of Biochemistry, Capital University of Medical Sciences;2. National Food Research Institute, Ministry of Agriculture Forestry and Fisheries
  • Received:2001-11-20 Revised:1900-01-01 Online:2002-04-15 Published:2002-04-15

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Abstract: The P-hydroxybenzoate 3-hydroxylase(POB-HOase)with 45 thousand relative molecular mass from Comamonas testosteroni was purified by using sonic crushing, ammonium sulfate fractionation, molecular sieve chromatography, exchange chromatography and SDS-PAGE. The N-termina lamino acid sequence was determined up to 21 residues. The amino acid sequence was compared with the known N-terminal sequences of POB3-HOases from Pseudomonas aeruginosa, Pseudomonas fluorescens and Acinetobacter calcoaceticus. The high degree of homology observed between the POB3-HOases from Comamonas and those of Pseudomonas and Acinetobacter indicates the common evolutionary origin of POB3-HOase among these soil bacteria of different genera.

Key words: para-hydroxybenzoare 3-hydroxylase|purification|evolutionary origin|Comamonas testosteroni

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