Table of Content

15 April 2002, Volume 23 Issue 2

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基础研究

^99mTc Labeled Monoclonal Antibody for Localization of Venous Thrombus

Dong Xiaoli;Li Liang;Song Aili;He Shida;Zhang Shunying;Wang Jue;Chen Ruifen;Song Lina

2002, 23(2):  97-100. 

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To evaluate the precious localization of venous thrombus targeted by monoclonal antibody, anti-D-dimer antibody was labeled with ^99m Tc and administrated into femoral vein of the rabbits that were subjected the venous thrombus. By radioimmunoimaging technique, the distribution, blood flow and radio-specificity of ^99m Tc-anti-D-dimer within thrombus were analyzed both in intact and thrombolized animals. ^99m Tc-anti-D-dimer was specifically concentrated in thrombus. The increase in blood flow was clearly observed in venous thrombus. In addition, no abnormal reaction resulted from the injection of ^99m Tc-anti-D-dimer and pathologic changes in various organs were found. It is concluded that the ^99m Tc-labeled anti-D-dimer antibody can be one of potential tools for experimental thrombus diagnose.

Preliminary Purification and Pursuit of Evolutionary Origin of P-hydroxybenzoate 3-hydroxylase from Comamonas Testosteroni

Chen Rui;Hosokawa Keiichi

2002, 23(2):  101-104. 

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The P-hydroxybenzoate 3-hydroxylase(POB-HOase)with 45 thousand relative molecular mass from Comamonas testosteroni was purified by using sonic crushing, ammonium sulfate fractionation, molecular sieve chromatography, exchange chromatography and SDS-PAGE. The N-termina lamino acid sequence was determined up to 21 residues. The amino acid sequence was compared with the known N-terminal sequences of POB3-HOases from Pseudomonas aeruginosa, Pseudomonas fluorescens and Acinetobacter calcoaceticus. The high degree of homology observed between the POB3-HOases from Comamonas and those of Pseudomonas and Acinetobacter indicates the common evolutionary origin of POB3-HOase among these soil bacteria of different genera.

Study of Progesterone Receptor in Extracted Tissue after Mifepristone Induced Abortion

Liang Yuanjing;Xu Qing;Zhu Dingshou;Fang Jingyan 

2002, 23(2):  105-106. 

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It is reported that remaining decidua and villi are found in the uterus of some patients who have uterogenic bleeding after mifepristone induced abortion. The purpose was to study the expression of progesterone receptor(PR)in these remaining tissues(n=19)by immunocytochemical LSAB methods. PR was mainly distributed in glandular epithelium, peripheral musculodermic cells, endometrial stroma cells, vesicular endothelium and musclar cells in uterus. These tissue showed positive or strong positive. The remaining chorion villi showed weak positive. Decidual showed negative, despite a few positive cells that were extra-chorion trophoblast and vescular endothelium. PR in the endometrium and decidua were significantly different(P<0.01). This study indicates that the residue of decidua and villi after mifepristone induced abortion are not caused by PR in these tissues.

Synthesis and Structure-relationship of a New Kind of Inhibitor of Mercapto-proteinases

Chen Gaiqing;He Jie;Zhang Feng;Li Yuying;Li Yulan

2002, 23(2):  107-109. 

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A new kind of antibiotics containing Tetrahydro-2H-1,3,5-thiadiazine-2-thione(THTT)was studied. According to the basic structure-relationship of THTT, the antimicrobial activity is mainly depended on the C₃-substituents. The structure of these compounds were confirmed by UV, IR, H-NMR and elemental analysis. The in vitro activity of the compounds against 6 kinds of bacteria was tested. The antimicrobial activities of all compounds were more potent than sulfadiazine sodium, especially the benzal-substituent THTT, which suggest that it may be worth studying further.

Observation of SP and CGRP Immunoreactive Characteristics in Gallbladder of Guinea Pig with Electron Microscope

Su Hongxing;Tetsuo Inokuchi;Masao Hamasaki

2002, 23(2):  110-112. 

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To investigate the biological behavior of the gallbladder, observed the histological pattern of nerve fiber, neuron and mast cell by using the ABC immunohistochemical method. Light electron microscope was used in order to observe synaptic vesicle in the nerve fiber of guinea pig gallbladder, a number of large dense cored vesicles and a few small undense cored vesicles were seen in the nerve fiber containing substance P and calcitonin gene-related peptide(CGRP)positive immunoreactivity. The experiment also showed that around the blood vessel and lymph vessel the synaptic vesicles of positive immunoreactivity nerve fiber were small dense cored vesicles.

Changes of SP and VIP in Blood and Ileum of Cool Stress Rats

Li Lisheng;Qu Ruiyao;Guo Hua;Wang Wei;Meng Yang

2002, 23(2):  113-114. 

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Substance P(SP)and vasoactive intestinal peptide(VIP)levels in blood and all layers of initial part of ileum in rats were measured by radioimmunoassays(RIA)in order to explore the mechanism of cool stress. SP levels in ileum of cool stress didn't change while the VIP levels in ileum of cool stress were significantly lower than the controls groups(P<0.05). SP levels in blood of cool stress were significantly higher(P<0.01)while the VIP levels in blood of cool stress were significantly lower than the controls groups(P<0.01). The results shows that changes of SP and VIP levels in the initial part ileum and blood of cool stress may be related to the gastrointestinal motility disorders presented in cool stress rats.

临床研究

Effect of APP17 Peptide on the Hippocampal Neurons Ultrastructure and Expression of InsR, Ins-1 in Type 2 Diabetic KKAy Mice

Zhuang Xiaoming;Zhao Zhiwei;Ji Zhijuan;Zhao Yongmei;Sheng Shuli 

2002, 23(2):  115-118. 

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The objective of the study was to investigate the expression of apoptotic-related proteins in hippocampal neurons of KK mice and the hippocampus ultrastructure in diabetic KK mice. KK mice were divided into three groups: normal control group, diabetic group and diabetic group treated with APP17-mer peptide. After 12 weeks, crystat sections of mouse brain were prepared and immunohistochemically stained for InsR, IRS-1. Three groups of the mice were observed and analyzed by electron microscopy. The levels of blood glucose and plasma insulin in DM group and DM+APP17P group were significantly increased and compared with C group. There were no significant difference between DM group and DM+ APP17P group. The expression of InsR, IRS-1 significantly decreased in diabetic mice as compared with normal controls(P<0.05), and find mitochondria edema in hippocampus in DM group. APP17 peptide may activate the signal transductive pathway. It may also improve the apoptosis of neuron in type 2 diabetic KK mice.

Treatment of Craniopharyngiomas by Modified Pterional Approach

Qi Wei;Zhang Maozhi;Zhao Jizong;Wang Lei;Wang Rong

2002, 23(2):  119-121. 

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To evaluate the results and significance of a modified pterional approach, 20 patients with craniopharyngiomas were operated by using this approach. The surgical results were analyzed prospectively and compared with the standard pterional approach. It showed that no significant difference in the tumor resection degree and main complications between two approaches(P>0.05). But patients treated by the modifed approach had less operative trauma, achieved better out-looking and had shorter hospitalization(P<0.05). The modified approach is a safe and convenient minimal-invasive approach in the treatment of lesions of the sellar region such as craniopharyngiomas.

Curative Analysis of Surgical Treatment for Young-onset Parkinson Disease

Ma Kai;Li Yongjie

2002, 23(2):  122-124. 

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In this study 343 patients with Parkinson disease underwent microelectrode guided stereotactic surgery from July, 1998 to December, 1999. These people were divided into two groups, 47 cases in young-onset Parkinson disease group(YOPD)and 296 cases in regular Parkinson disease group(PD). United Parkinson Disease Rating Scales(UPDRS)were used to judge patients' performance before and after the operation during "on" and "off" states. These data were used to find out if there was any difference concerning the surgical results between YOPD group and PD group. For each group, there was a significant difference concerning UPDRS before and after the operation either during "on" or during "off" states. Furthermore, when the illness history was within 10 years, YOPD group's performance in the third part of UPDRS is significantly better than PD group. When the illness history is longer than 10 years, YOPD group's performance in the second part of UPDRS significantly better than PD group.

Gene Expression of Peripheral Blood from a Heart Graft Patient

Xu Xiufang;Li Wenbin;Meng Xu;Li Wen;Liu Yanxia;Liu Shu;Zhou Jian;Huang Yimin

2002, 23(2):  125-127. 

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In the paper the technique known as differential display polymerase chain reaction(DD-PCR)was applied to explore genes expression in white cells of peripheral blood drawn from an allogenic heart graft patient on the day before the transplant operation and after the operation in order to assess genes′role in transplantation. Morphologic changes of the heart grafts were observed 11 d after transplant operation. RPL23 gene was expressed undulatority after the operation, but strongly when acute rejection occured; FK506 binding protein 1A gene was expressed weakly after the operation,but strongly when acute rejection occured. The result indicates that the study of genes expression of peripheral blood from a heart graft patient will be useful to assess genes′role in transplantation

Expression of Fas Antigen on Bone Marrow CD34⁺ Cells in Patients with Aplastic Anemia

Wan Suigui;Sun Xuejing;Liu Congyan;Zhao Hong;Xu Juan;Tian Ding

2002, 23(2):  128-130. 

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The objective of the study was to investigate Fas antigen induced apoptosis in the pathogenesis of aplastic anemia. The expression of Fas antigen on bone marrow CD34⁺ cells and the effect of IFN-γ to it were assayed in AA by flowing cytometry and short-liquid-culture in vitro. Fas antigen expression on CD34⁺ cells in SAA and CAA was significantly higher than that in normal controls 〔(38.28± 9.01)% and(26.66±4.27)% vs(10.02±2.33)%, (P<0.01, (P<0.05 respectly〕. Fas antigen expression on CD34⁺ cells in CAA was significantly lower than that in SAA(P<0.05). Addition of IFN-γ to liquid culture of BMMNC including SCF, IL-3, GM-CSF and EPO cytokines showed significant stimulating effects on Fas antigen expression in AA, but not in normal controls, the expression in SAA was higher than that in CAA(P<0.05). The abnormal expression of Fas antigen on CD34⁺ cells may be involved in the pathogenesis of AA.

Effects of Calcium Gluconate on Changes of Coagulation during Hypervolemic Hemodilution Induced by Gelofusine

Ji Yong;Wang Huiwen;Wang Baoguo;Wang Aidong;Zhang Tong

2002, 23(2):  131-134. 

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To compare the changes of coagulation during hypervolemic hemodilution induced by gelofusine(G)or gelofusine containing calcium gluconate(calglucon), 30 neurosurgical patients without heart diseases were randomly allocated into three groups. Hypervolemic hemodilution was induced by rapid infusion, 25 mL/min, of gelofusine(groupⅠ, n=10), gelofusine containing calglucon(0.5 g in 500 mL, groupⅡ, n=10)and of gelofusine containing calglucon(1.0g in 500mL, groupⅢ, n=10)after general anesthesia. Hematocrit(HCT), activate partial thromboplastin time(APTT), prothrombin time(PT)and calcium concentration in serum(Ca²⁺)were tested after anesthesia induction, gelofusine infusion of 500 mL, 1000 mL, 1500 mL and 30 min after infusion, respectively. During hypervolemic hemodilution, PT and APTT were prolonged more in groupⅠthan in groupⅡ and groupⅢ. In groupⅠ, PT and APTT were prolonged by 13.9% and 21.8%, respectively. PT and APTT were prolonged by 10.6% and 12.4%, respectively in groupⅡ, and were prolonged 9.8% and 8.2%, respectively in groupⅢ. Ca²⁺ decreased by 13.8% in groupⅠ, but it did not change significantly in groupⅡ, and increased by 6.4% in groupⅢ, after infusion of 1500 mL gelofusine. The result shows that gelofusine is suitable for hypervolemic hemodilution, but it leads to coagulation disorder which is related to the decreased serum Ca²⁺. Adding 0.5 g calglucon to 500 mL of gelofusine can partly prevent the depression of coagulation.

Detection and Significance of c-myc Protein in Invasive Breast Carcinoma

Zhang Dong;Gao Dongchen;Zhang Changhuai;Lu Xiaomei;Chen Shuyuan

2002, 23(2):  135-138. 

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To investigate the clinical significance of c-myc protein expression in breast cancer, the expression of c-myc protein in 116 breast cancer tissues was examined using immunohistochemical methods(SP). Nuclear expression of c-myc protein was detected in 11.2% of tumors, and it was related to prognosis. Cytoplasmic expression of c-myc protein was present in 93.1% of the carcinomas. Moderate to strong c-myc protein expression in cytoplasmic was associated with the positive status of ER. The result shows that nuclear expression of c-myc protein appears to be an important prognostic factor. To investigate the expression of c-myc protein in breast cancer may to some extent act as guidance to the therapy of breast carcinomas.

Effects of Salt on Levels of Angiotensin Ⅱ in Plasma and Tissues

Xu Xiuying;Huang Yan;Wang Jiarui

2002, 23(2):  139-141. 

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To investigate the effects of salt on the levels of angiotensin Ⅱ(AngⅡ)in plasma and tissues in spontaneously hypertensive rat(SHR), twenty 8-week-old male SHRs were divided into experimental and control groups(n=10). They were given 2% NaCl and distilled water respectively for 6 weeks. Plasma renin activity(PRA)and AngⅡ levels were measured by radioimmunoassays. Results showed that PRA in experimental group was decreased(1.13±0.72)ng/(mL·h)vs(1.94±0.96)ng/(mL·h, (P<0.05)and plasma AngⅡ had a slight decrease but no significant difference 〔(89.4±1.4)ng/L vs(111.6± 1.3)ng/L, P>0.05〕 compared with the control. AngⅡ levels increased in heart and artery in the experimental group 〔(227.1±1.1)pg/g vs(151.4±1.1)pg/g, (P<0.01,(57.1±1.5)pg/g vs(30.7±2.0)pg/g, (P<0.05〕, AngⅡ levels in kidney appeared to be no significant difference between two groups. The experiment indicates that circulatory and tissue renin-angiotension-system response to salt was different in SHR.

Relationship between Myocardial Viability and the Presence of Arrhythmia in Patients with Old Myocardial Infarction

Du Fenghe;Xu Xiuying;Zhao Lijia;Yang Honglian

2002, 23(2):  142-144. 

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To investigate the relationship of myocardial viability to the presence of arrhythmia in patients with old myocardial infarction. Ninety-eight patients with previous infarction were studied. Myocardial viabilitis were detected by ^99mTc MIBI myocardial tomography with nitroglycerin administration. The presence of arrhythmias was detected by 24 hours ambulatory electrocardiography. Fifty-five patients(56.1%)had improvement of perfusion after nitroglycerin administration, among them, arrhythmias were found in 32 patients(58.2%), comparing 11 patients(25.6%)with arrhythmias in the group without myocardial viability(P<0.01). The presence of viable myocardial in patients with old myocardial infarction have a higher frequency of arrhythmias.

Famciclovir in Treatment of Patients with Chronic Hapatitis B Virus Infection

Chen Xinyue;Min Jia;Li Junhong;Xin Yongmei;Wang Juntao

2002, 23(2):  145-147. 

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The objective was to evaluate the effect of famciclovir on the decrease of serum hepatitis B virus(HBV)DNA and ALT levels in chronic hepatitis B patients and its safety profile and tolerance compared with the contrast. Fourty chronic hepatitis B patients defined by positive HBsAg, HBeAg and HBV DNA were enrolled and randomized into famciclovir and contrast groups for 16 weeks. The efficacy and safety were evaluated with clinical, biochemical and virological parameters. At the end of 16 weeks, patients with elevated ALT levels at baseline became normal by 65% in the famciclovir treated group as compared with the contrast group where only 35% were normal(P<0.01). And in 60% of famciclovir treated patients HBV DNA levels showed significant decrease compared with 10% of those contrast patients(P<0.01). In 6 patients of FCV group HBeAg became negative, which didn't occur in the contrast group(P<0.05). Famciclovir was well tolerated in a dose of 500 mg three times daily and the overall incidence of adverse effects was similar to the contrast. Most adverse events were mild. Famciclovir is very effective in the inhibition of HBV replication, and often decreases serum ALT levels. Famcoclovir is well tolerated without severe adverse effects during the treatment.

Analysis of Death Causes for AIDS Patients

Zhang Tong;Wu Hao;Zhang Ke;Xue Lianzhi;Sun Guizhen

2002, 23(2):  148-150. 

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The objective was to comprehend the current situation of death causes for the patients of Acquired immunodeficiency syndrome(AIDS.)All the cases died in You′an hospital, and were analysed in the aspects of their episode progress, death progress(course), complication and treatment etc. Latent period of these patients was(8.00±5.72)years, the average death period(2.42±7.24)months. CD4⁺T lymphocytes counting was(31±25)/mm³, virus load(46 700±7 630)copy/mm³. Multiple opportunity infection and tumor were combined at the same time, which led to the patients death in late period. Common complications, for example pneumocystis carinii pneumonia(PCP), pulmonary tuberculosis, mouth cavity and esophago Monilia infection seldom led to death, but invaded the central nervous system,(for example tubercular meningitis, cryptococcal meningitis, and HIV encephalopathy)or accompanying severe infection. Malignancy Tumor prognosi is very bad.

Effects of Berberine on Patients with IBS

Wang Yanbin;Tang Xiaoshan;Yang Zhaoxu

2002, 23(2):  151-152. 

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To evaluate the efficacy of berberine on the patients with IBS, ninty-nine patients with IBS were divided into 3 groups and administered with bererine(200 mg, tid), cerecilon(100 mg, tid)and placebo(vitamine Bco 2 tab, tid)for a 14 day-course respectively. The result showed that the berberine could thereby contribute to improvement in the symptoms of IBS, especially for diarrhea. The total rate of effectiveness of bererine group was 70%, which showed a statistically significant difference(P<0.01). No side effects attributable to the drug were encountered. Bererine is effective and safe in treating IBS patients. Another advantage of the agent is its low cost.

Anti-VEGF Monoclonal Antibody Inhibit the Growth of Osteosarcoma

Cai Youbo;Zhang Wei

2002, 23(2):  153-155. 

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In this study, the availability of specific monoclonal antibodies capable of blocking VEGF-induced angiogenesis in vivo allowed us to test the hypothesis directly. The aim of our study was to observe the inhibition of growth of osteosarcoma by anti-VEGF monoclonal antibody through animal experiment. We also investigate the effect of anti-VEGF antibody on microvessel density in tumors through histological method. Twenty-three female BALB/C nude mice were used in this study. OS-732 osteosarcoma cells were injected into the armpit subcutaneous part(1070/mL for each mouse). The nude mice are divided into four groups randomly(A, B, C, and D). The anti-VEGF monoclonal antibody was injected into the six mice of group A at the next day of the osteosarcoma cell-injection. Two weeks later, the antibody was injected into the six mice of group B. PBS was injected into the six of group C and nothing to the five of group D at the same time with group A. The single application of the antibody was 200 μg for each mouse three times weekly for three weeks. The mice of group A and C were killed four weeks later and group B and D six weeks later. Every mouse was anatomized and the tumor was taken out for a pathological slice. The tumor size and counted the microvessel density were meesured. Comparisons of the results with and without the treatment were performed using a Student T-test. A value of (P<0.05 was considered significant. The tumor growth was significantly inhibited only when the anti-VEGF antibody administration was started immediately after tumor cell inoculation(group A)but not when such treatment was initiated two weeks later(group B), when the tumors were already established. When comparing the microvessel density, there were no significant differences between the four groups. In summary, our results demonstrated, for the first time, that the incipient growth of osteosarcoma could be inhibited significantly by anti-VEGF antibody. The anti-VEGF antibody may be useful for the prevention and clinical treatment of the metastasis focus of osteosarcoma.

Experience of Construction of Canine Aneurysnal Models with Fransplantation of Venous Pouches

Yi Ke;Ding Yanling;Wu Zhongxue;Yang Xinjian

2002, 23(2):  156-158. 

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The research explored the methods of production of canine models of lateral, bifurcation and terminal arterial aneurysms. Aneurysmal models were constructed by anastomosing venous pouches onto the rebuilt common carotid arteries under general anesthesia. The experimental aneurysms were evaluated by digital subtraction angiography(DSA)after 1 to 3 weeks. Fifteen lateral aneurysms, 9 bifurcation aneurysms and 8 terminal aneurysms were obtained Successfully. One animal died of over-dosed anesthesia. Another died from rupture of a terminal aneurysm. One dog had its bifurcation aneurysm, lateral aneurysm and parent arteries completely thrombosed, 3 had their lateral aneurysms occluded spontaneously, and one had its terminal aneurysm ruptured to form pseudoaneurysm 3 weeks after operation. The results demonstrate that construction of aneurysmal models by anastomosing the venous porches is a ideal method with a highly successful rate of surgery and fewer complications.

Clinical Analysis of 36 Cases of Severe Trauma Complicated with Disseminated Intravascular Coagulation

Wang Peilin;Yu Wei;Bai Rixing

2002, 23(2):  159-161. 

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To investigate the clinical and experimental features of trauma complicated with disseminated intravascular coagulation(DIC)and to improve the level of diagnosis and treatment to trauma complicated with DIC, 36 cases of trauma complicated with DIC from 1980 to 1998 were analysed. All the cases were severely injuried, among which 4 cases were in early stage of DIC and 32 cases were in late stage; 28 cases complicated with infections, 25 with shock and 10 with ARDS. The primary clinical features were repetitive and multiple hemorrhage in the whole body, refractory shock or multiple organ malfunction. All the cases showed the progressive reduction of Plt, prolongation of PT and 3P positive. Twenty- five cases were treated with low-dose heparin, 9 of the 25 cases died, the morality was 36%. The most important predisposing factors leading to DIC in severe trauma are infection and shock. The continuous reduction of Plt, prolongation of PT and positive of 3P are important to the diagnosis of DIC. ARDS incidence is high in traumatic DIC. The use of low-dose heparin may improve the treatment result.

Mechanism of Humoral Factor-leukocyte on Lung Injury in CHD with Severe Pulmonary Hypertension during Perioperation

Zhang Hong;Zhou Qiwen;Wen Shaojun;Meng Xu

2002, 23(2):  162-165. 

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To investigate the mechanism of the humoral factor-leukocyte on lung injury in congenital heart disease(CHD)with severe pulmonary hypertension during perioperation, 31 consecutive cases of CHD with ventricular septal defect(No pulmonary hypertension 16 case, severe pulmonary hypertension 15 case)were studied during perioperation. Mean pulmonary arterial pressure, pulmonary vascular resistance, TXB₂, 6-keto-PGF₁α, MDA, IL-8, leukocyte and neutrophilic granulocyte number were measured. TXB₂,the rate between 6-keto-PGF₁αand TXB₂(P/T), MDA,IL-8, leukocyte and neutrophilic granulocyte number were closely related to pulmonary pressure,pulmonary vascular resistance and respiration index(RI)in the case of CHD with severe pulmonary hypertension during perioperation. The humoral factor-leukocyte of the cases of CHD with pulmonary hypertension changed significantly during perioperation, which led easily to pulmonary damage. Lung injury during perioperation may affect hemodynamics.

Artificial Joint Replacement in Fifteen Patients with NIDDM

Tian Baopeng;Zhang Zezheng;Wang Bao

2002, 23(2):  166-168. 

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Fifteen artificial joint replacement were performed for fracture of the femoral neck in fifteen patients who had NIDDM(non-insulin depend diabetes mellitus). The results were excellent in two years follow-up. There were no complications. Authors consider that artificial joint replacement should be the treatment for NIDDM patients with fracture of the femoral neck. The careful management in the peri-and post-operation is important.

Upper Urinary Epithelial Cell Carcinoma

Su Yong;Zhou Yongjian;Han Zhixing

2002, 23(2):  169-170. 

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To study the clinical feature diagnosis and treatment of upper urinary epithelial cell carcinoma, 42 cases of upper urinary transitional cell carcinoma as a result of pathological verification within 10 years were reviewed and analyzed. And summary and comparison were made in progress for its clinical feature, urine cytology examination results, the examination of image feature and cystoscope. Eleven cases had multi-organ relapse in the meantime, 40 cases of patients were operated, 34 cases of patients were followed up postoperatively. Urine cytology examination positive rate was 66.7%, and it could reach 92.9% after irrigation of retrograte intubation. CT positive rate was 85.7%. Six cases had bladder tumor relapsed after operation. Urine Cytology examination is the idiosyncratic qualitative diagnosis method, the pyelic Urine can raise the positive rate, CT examination has important value in orientation and stage of cancer. Multi-organs trend should be handled seriously, the treatment should be radical excision mainly, and the cystoscope examination should be carried out routinely.

Effects of EGF on Proliferation and Alkaline Phosphatase Expression of Human Periodontal Ligament Cells

Lü Ying;Zhang Haiyan;Liu Xiaoyong;Li Weihong 

2002, 23(2):  171-173. 

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The aim of the study was to evaluate the biological effects of epidermal growth factor(EGF)on the proliferation and alkaline phosphatase(ALP)activity in cultured human periodontal ligament cells(PDLCs). Thiazoly blue(MTT)colorimetric assay and ALP activity analysis were used at different time points(24,48 and 72 h)to determine the proliferation and ALP activity of PDLCs after different doses of EGF were added into the culture medium. During 24～72 h, when compared with control group, 0.1～5.0μg/L EGF significantly inhibited the proliferation of PDLCs((P<0.01～0.05); 10 μg/L EGF significantly stimulated the proliferation of PDLCs at 48 h(P<0.05)and stabilized the proliferation of PDLCs at 72 h(P>0.05); 0.1～0.5μg/L EGF significantly decreased the ALP activity of PDLCs((P<0.01～0.05); 1～10μg/L EGF significantly increased the ALP activity of PDLCs((P<0.01～0.05)at 24 h and stabilized the ALP activity of PDLCs at 48 h and 72 h(P>0.05). These results suggest that 10 μg/L EGF may simultaneously promote the proliferation and differentiation of human PDLCs during a period of time.