Clonging, Expression and Biological Activity of Human Insulin-like Growth Factor-1

Journal of Capital Medical University ›› 2004, Vol. 25 ›› Issue (2): 154-158.

• 论著·基础研究 • Previous Articles Next Articles

Clonging, Expression and Biological Activity of Human Insulin-like Growth Factor-1

Qi Yahui¹, Wang Yamei¹, Sun Licui¹, Si Yang¹, Yan Yudong¹, Zhang Jinyi¹, Bing Guoying²

1. Experiment Center, Capital University of Medical Sciences;2. Department of A natomy and Nerobiology, University of Kentucky USA

Received:2003-11-21 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

Abstract

Abstract: The human insulin-like growth factor-1(IGF-1) cDNA was amplified by RT-PCR from human tonsil tissue. Sequenced, the IGF-1 cDNA was inserted into eukaryotic expression vector pcDNA3.1(+). The recombinant plasmid pcDNA/I was transferred into HUVEC cells mediated by liposome. The activity of IGF-1 was detected by MTT. The results showed that IGF-1 protein was expressed in HUVEC cells 72 h after gene transference and it had good biological activity to stimulate HUVEC proliferation. pcDNA/I was transferred into 293 cells mediated by liposome. Screened with G418, we got positive cell which can express recombinant protein of IGF-1 stably. Chick charioallantoic membrane(CAM) bioassay show that the recombinant protein has biological activity to promote blood vessel formation.

Key words: insulin-like growth factor-1, gene clone, gene expression

CLC Number:

Q785

Qi Yahui;Wang Yamei;Sun Licui;Si Yang;Yan Yudong;Zhang Jinyi;Bing Guoying. Clonging, Expression and Biological Activity of Human Insulin-like Growth Factor-1[J]. Journal of Capital Medical University, 2004, 25(2): 154-158.

[1]	Zhang Jianxiong, Li Dandan, Hu Mengmeng, Cheng Xuewei, Dong Ruihua. Relationship between carboxylesterase 1 and carboxylesterase 2 gene expression levels and body mass index, and gene polymorphism analysis [J]. Journal of Capital Medical University, 2023, 44(1): 13-19.
[2]	Yao Jiannan, Gao Tianbo, Duan Ling, Liu Jian, Jiang Yuliang, An Guangyu, Ge Yang. Screening and analysis of O-glycosylation-associated differential expressed genes in colorectal cancer [J]. Journal of Capital Medical University, 2020, 41(3): 403-410.
[3]	Xu Wenjian, Li Wei. Building a prediction model of brain tissues gene expression based on whole blood gene expression profiles [J]. Journal of Capital Medical University, 2019, 40(5): 731-737.
[4]	Ning Junyu, Jing Haiming, Du Hongju, Qi Lijuan, Gao Shan, Li Guojun. AMP-activated protein kinase inhibits insulin-like growth factor-1 signaling in vascular smooth muscle cells via regulating activation of extracellular signal-regulated kinases 1/2 [J]. Journal of Capital Medical University, 2019, 40(1): 101-105.
[5]	Niu Huan, Cheng Shan, Ding Wei. The 2018 Lasker Awards(Ⅰ)-Basic Medical Research and Special Achievement in Medical Science [J]. Journal of Capital Medical University, 2018, 39(5): 778-782.
[6]	Fa Pengyan, Liu Hua, Zheng Junfang, Wang Lei, Dai Yuanping, He Junqi. NHERF3 expression level and its significance in renal cell carcinoma [J]. Journal of Capital Medical University, 2015, 36(2): 251-254.
[7]	REN Hai-ying;ZHAI Jian-jun;GAO Hong. The influence of human serum insulin-like growth factor-1 on the endometrium in female breast cancer with tamoxifen treatment [J]. Journal of Capital Medical University, 2012, 33(5): 594-596.
[8]	XING Feng-ling;CHEN Xue-qin;FENG Li-min;ZHANG Hua. Expression of Tumor Suppressor Gene PTEN in Endometrium of Postmenoposal Women with Breast Cancer Treated with Tamoxifen [J]. Journal of Capital Medical University, 2010, 31(3): 405-408.
[9]	MENG Xiang-hong;ZHAO Zhi-wei;JI Zhi-juan;ZHANG Jing-yan;WANG Rong. Effect of Analog P165 of APP 5 mer Peptide and Rosiglitazone on Change of Spatial Reference Learning and Memory Ability in Type 3 Diabetes Mellitus(T3DM) Rats [J]. Journal of Capital Medical University, 2010, 31(1): 65-68.
[10]	DING Hui;CAI Yan-ning;WANG Ling-ling;CHAI Yu-xin;ZHANG Yan-li;CHEN Biao. Rhythmic Oscillation of Neurotransmitter-related Genes Expression in the Mouse Striatum [J]. Journal of Capital Medical University, 2009, 30(5): 580-583.
[11]	XU Min;JIA Jian-ping;GENG Zhi-wei;SONG Jue-xian;WU You;LI Hao;LIU Lu;LIU Fang-yan. Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons [J]. Journal of Capital Medical University, 2009, 30(4): 516-520.
[12]	Wang Yamei;Li Shentao;Weng Mingjie;Sun Licui;Si Yang;Yan Yudong;Qi Yahui. Expression of Human Vascular Endothelial Growth Factor in Cultured NIH3T3 Cells [J]. Journal of Capital Medical University, 2008, 29(6): 737-740.
[13]	Wang Peichang;Zhang Jian;Zhang Zongyu;Tong Tanjun. The Effects of HDTIC Compounes Isolated from Astragali Radix on the Expression of p16 and p21 [J]. Journal of Capital Medical University, 2007, 28(2): 145-149.
[14]	Wang Yamei;Sun Licui;Yan Yudong;Si Yang;Zhang Jingyi;Qi Yahui. Expression of Human Basic Fibroblast Growth Factor Gene in Baculovirus-Insect Expression System [J]. Journal of Capital Medical University, 2007, 28(2): 229-233.
[15]	Li Li;Gao Xiulai;Song Yizhi;Lu Tao;Jing Peng . Reconstruction and Expression of Regucalcin Fusion Protein in E.coli [J]. Journal of Capital Medical University, 2006, 27(6): 788-791.

Clonging, Expression and Biological Activity of Human Insulin-like Growth Factor-1

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments