Abstract:

Objective To study the sequence of F protein gene of 4 respiratory syncytial virus strains in Beijing area of China.Methods P₁ and P₂ segments of respiratory syncytial viruses were amplified by RT-PCR,then the PCR products were purified for sequencing.Analysis was carried out by Bioedit program.Results The nucleotide sequences of the four RSV strains F mRNA had 94.92%～95.62% overall nucleotide identity with those of RSV isolate A₂,the nucleotide sequences of signal peptide of four RSV strains F mRNA had 99.36%～99.73% identity,the F1 chain had 98.93%～99.31%,F2 chain had 96.69%～97.33% identity respectively.The deduced aminoacid sequences of the four strains and A₂ F proteins had 97.39%～97.74% overall aminoacid identity,the signal peptide had 99.13%～99.65%,the F1 chain had 98.78%～99.48%,F2 chain had 98.61%～99.30% identity respectively.Conclusion The differences between the A₂ and Beijing strains in the F gene are small,so the nucleotide and aminoacid have high identity.The antigenic area Ⅱ could be the potential site of RSV vaccine.

Key words: respiratory syncytial virus, F protein, nucleotide sequence