Screening Genes Associated with Biological Signal Conduction and Immunity in Lung Squamous Cell Carcinoma by BiostarH-Ⅰ cDNA Microarray

Abstract

Abstract: Objective By use of cDNAmicroarray, to screen out the differential expression genes which are related with biological signal conduction, humoral or cellular immunity in lung squamous cell carcinoma and normal lung tissue.Methods The total RNAs were isolated from tumor and normal lung tissue and were reversely transcribed to prepare the hybridization probe.The mixed probes were hybridized with the BiostarH-Ⅰ cDNAmicroarray.After washing, the cDNAmicroarray was scanned for the fluorescent signals by ScanArray 4000 scaning apparatus.The scaning data was analyzed by Genepxix Pro software and differential expression genes between these two kinds of tissues were shown out.Results Eight strips of genes were screened out.Among them, five genes'expression level were up-regulated(r>2.0), including: METAP2, LIV-1, CUL2, CSE1L, RBBP2H1A, and the rest three strips were down-regulated(r<0.5), including: HBB, CKB, CX3CL1.Conclusion cDNAmicroarray can realize high-through out screening of the genes which associated with lung cancer.Eight genes which related with biological singal conduction or immunity in lung squamous cell carcinoma are found.Further investigation about these genes will be carried out later.

Key words: cDNA microarray, lung cancer, immunity, biological signal conduction

CLC Number:

R734.2

Bai Peng;Zhang Husheng;Wang Yuzhou. Screening Genes Associated with Biological Signal Conduction and Immunity in Lung Squamous Cell Carcinoma by BiostarH-Ⅰ cDNA Microarray[J]. Journal of Capital Medical University, 2005, 26(6): 729-732.

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Screening Genes Associated with Biological Signal Conduction and Immunity in Lung Squamous Cell Carcinoma by BiostarH-Ⅰ cDNA Microarray

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