Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (4): 423-426.

• 专题报道 • Previous Articles     Next Articles

Expression of the Adeno-associated Virus Rep78 as a Major Regulatory Protein and Its Binding with Hepatitis B Virus X Gene Promoter in Vitro

Liu Tianhui, Cong Min, Wang Ping, Tang Shuzhen, Wang Baoen, Jia Jidong, You Hong   

  1. Liver Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences
  • Received:2006-06-16 Revised:1900-01-01 Online:2006-08-24 Published:2006-08-24

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Abstract: Objective This study is to investigate the possible effect of adeno-associated virus(AAV) Rep78 on hepatitis B virus X gene(HBV-X).Methods Rep78 protein was produced as a fusion protein with MBP using pMAL-Rep78 plasmid(with full gene of Rep78) in DH-5α.SDS-PAGE and western blot were used to confirm the expression of MBP-Rep78.Electrophoretic mobility shift assay(EMSA) was utilized to detect the binding of MBP-Rep78 protein with HBV-X promoter.Results Purified MBP-Rep78 fusion protein was obtained successfully.EMSA showed Rep78 protein could bind with HBV-X promoter strongly in a dose-dependent manner.Conclusion AAV Rep78 could bind with HBV-X promoter.

Key words: adeno-associated virus, hepatitis B virus, Rep78, electrophoretic mobility shift assay

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