Abstract: Objective To investigate the biological characteristics of cell lines established from healthy and diseased human dental alveoli.Methods We first established primary cell lines from either healthy or diseased or human dental alveolus.Two cell lines,H-258 and H-171,respectively derived from healthy or diseased tissues,were selected for further studies on cell morphology,growth and aging by cell counting,histochemical and immunohistochemical staining.Results Primary cell lines were successfully established from diseased human dental alveoli.Cell lines from healthy human dental alveoli were alive but with decreased growth speed.Both cell lines(H-171 and H-258) shared the characteristics with osteoblast as they showed an attached growth type and had positive staining with APK,tolduidine blue and BMP-2 ect.Conclusion Both cell lines shared the characteristics with osteoblast.Primary cell lines established from diseased human dental alveoli showed greater growth potential than that from healthy dental.

Key words: primary cell lines, chronic periodontitis, dental alveolus