Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (5): 607-611.

• 基础研究 • Previous Articles     Next Articles

Effects of Id4 on Apoptosis of K562 Cell

Liu Xin1, Zeng Shaoju2, Wang Yongchao2   

  1. 1. Department of Cell Biology and Genetics, School of Basic Medicine, Capital University of Medical Sciences;2. The Key Laboratory of Cell Proliferation and Regulation Biology, Beijing Normal University
  • Received:2006-03-22 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

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Abstract: Objective To study the effects of Id4 on cell apoptosis of humen erythroleukemia cell line K562.Methods The antisense Id4 gene was inserted into eukaryotic expression vector pXJ41-neo,the recombinant vector was transfected into K562 cells.The percentage of apoptotic cells induced by HT was analysed by flow cytometry,UNEL.Western blot for detection of the expression of Bcl-xl,c-Myc,p27. Results The cell apoptosis was most significant in pAId4 cells induced by HT,The expression of Bcl-xl was found down-regulated in pAId4 cells in comparison with the control.Conclusion The antisense Id4 gene was found to be a strong inducer of apoptosis in K562 cells.In addition,Id4 may be an upstream regulator expression of Bcl-xl,leading to apoptosis.

Key words: K562 cell, Id4 gene, cell apoptosis

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