Abstract:

Objective To investigate the effects of Fuzhengjiangan Formula(FZJGF) on collagen metabolism in liver fibrosis rat model induced by the human serum albumin. Methods The rat models with immune liver fibrosis were induced by the human serum albumin. Rats were treated with saline, FZJGF(crude drugs 9.85,39.4 g/kg, two dosage groups) and colchicine(0.000 1 g/kg). The contents of laminin, hyaluronic acid and collagen type Ⅳ in rat serum were measured with radioimmunoassay method. The level of hydroxyproline in liver tissue was detected with chemistry method. The pathological changes of liver tissue were observed with HE and Von Gieson staining. The contents of collagenous fiber were determined with image quantitive analysis system. Results FZJGF could significantly decrease the contents of LN, HA and Ⅳ-C. At the same time, it depressed the level of Hyp in rats liver. The pathological improvement of pathologyical changes were observed and FZJGF could markedly alleviate deposition of collagenous fiber, reduce liver pseudoluboli. The image quantitive analysis showed that the contents of collagenous fiber of liver significantly decreased after the treatment using FZJGF. Conclusion FZJGF could affect the collagen metabolism in liver to inhibit formation and development of rat hepatic fibrosis induced by the human serum albumin.

Key words: Fuzhengjiangan Formula, liver fibrosis, collagen, immune, human albumin, rat