Abstract: Objective Tumor-derived heat shock protein glycoprotein 96(HSP GP96) can be used as vaccines for refractory cancers. The effects of cytokines on the gene and protein expressions of GP96 in K562 cells at different concentrations and different hours were investigated. Methods K562 cells were coincubated with different concentrations of cytokines for 12 hours. GP96 mRNA and GP96 proteins of K562 cells were analyzed. Total RNA of K562 cells, which were respectively coincubated with concentrations increases, but they descend at certain concentrations(IFN-α 600 kU/L or IFN-γ 6 μg/L at different hours, were analyzed. Results 1) Dose-effect: changes of the gene and protein expressions of GP96 from K562 cells which were respectively coincubated with different concentrations of IFN-α and IFN-γ for 12 hours were similar: they gradually increased with the concentrations, but descended at certain concentrations(IFN-α 600 kU/L, IFN-γ 6 μg/L). 2) Time-effect: steady level of GP96 mRNA were achieved up to 9 hours of continuous exposure to IFN-α 600 kU/L and IFN-γ 6 μg/L, and then descended gradually. Conclusion IFN-α and IFN-γ can increase the protein expressions of GP96 from K562 cells by increasing gp96 gene expression.

Key words: IFN-α, IFN-γ, HSP GP96