Abstract: Objective To study the effect of transient receptor potential-canonical channel(TRPC) on MPP⁺-induced MN9D cell damage. Methods The MN9D cell damage model was induced by treating MN9D cells with different concentrations(62.5, 125, 250, 500, 1 000 μmol/L) of MPP⁺ for 24 h and with 500 μmol/L MPP⁺ for different time course(3, 6, 12, 24, 36 hours). The mRNA expression of the members of TRPC subfamily was investigated by RT-PCR and the protein levels of TRPCs by Western blot in MN9D cells. MTT was performed to detect TRPC3-mediated inhibition of MPP⁺ neurotoxicity in MN9D cells transfected by TRPC3-GFP and GFP adenovirus. Results 500 μmol/L MPP⁺ specifically reduced the protein levels of TRPC3 in MN9D cells and overexpression of TRPC3 protected MN9D cells from MPP⁺ neurotoxicity. Conclusion Degradation of TRPC3 induced by MPP⁺ was probably involved in pathogenesis of Parkinson's disease(PD) and this study maybe provide a new target to prevent and treat PD.

Key words: Parkinson's disease, MPP⁺, TRPC, neuroprotective effect