Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (1): 57-61.

• 鼻生理功能专题 • Previous Articles     Next Articles

Effect of ATP on Human Nasal Ciliary Beat Frequency

Shi Linggai1, Han Demin1,2, Zhang Luo1,2   

  1. 1. Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University;2. Beijing Institute of Otorhinolaryngology, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education
  • Received:2008-11-18 Revised:1900-01-01 Online:2009-02-21 Published:2009-02-21

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Abstract: Objective To investigate the effects of ATP on human nasal cilia movement. Methods Ciliary beat frequency(CBF) of cultured human nasal epithelial cells was measured by high-speed digital microscopy in ATP solution of different concentrations. Results CBF of cultured nasal epithelial cells exposed to HBSS showed no significant changes in 3 minutes(P>0.05); However in 1, 10 and 100 μmol/L ATP solution, CBF increased significantly in 10 seconds and reached the apex, then it showed a sustained elevation in 3 minutes. In 1 μmol/L ATP solution, CBF increased significantly from(9.2±1.8)Hz to(10.3±2.0)Hz(n=10)(P＜0.05). Similarly in 10 μmol/L ATP and 100 μmol/L ATP solution, CBF increased significantly when compared with samples in HBSS. Conclusion When 1, 10 and 100 μmol/L ATP were added to the chamber, there were rapid increase in ciliary activity. In addition, there is concentration-dependent relation between CBF and ATP.

Key words: ciliary beat frequency, human nasal epithelial cell, ATP

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