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Table of Content

    21 February 2009, Volume 30 Issue 1
    奖项介绍
    The Pilot of Chinese Neurosurgery——An Brief Introduction of the State Supreme Science and Technology Award Winner, Academician Wang Zhongcheng and His Major Scientific and Technological Achievements
    Cheng Xiaoyan;Wang Lingshu
    2009, 30(1):  1-4. 
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    At 10:00 on January 9, 2009, at the Great Hall of the People in Beijing, capital of China, in view of commend his predominant contributions to the diagnosis, treatment, scientific research and training of competent personnel for neurosurgery, Hu Jintao, General Secretary of the CPC Central Committee, President of the PRC and Chairman of the Central Military Commission, ceremoniously granted the 2008 State Supreme Science and Technology Award to Academician Wang Zhongcheng, who is Director of Beijing Neurosurgical Institute, Dean of Neurosurgical School of Capital Medical University, Honorary Director of Beijing Tian Tan Hospital. This article provides an overview of Academician Wang Zhongcheng's resume, chief contributions and its significance.
    The Second Grade National Prize for Science and TechnologyProgress——Systematic Study on Diagnosis and Treatment of Pulmonary
    Yang Yuanhua
    2009, 30(1):  5-7. 
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    This study was designed to improve the diagnosis and treatment and basic research work of PTE omnibearingly by improving recognition, standardizing the diagnosis and treatment and enhancing study. The guideline(draft) of pulmonary thromboembolism diagnosis and treatment was established, the delineation medicine, diagnostics, therapeutics, epidemiology and preclinical medicine of PTE in China were investigate and gained a series of achievements. The diagnostic and therapeutic system suited for China was established and was further generalized during the study; the delineation medicine data with large sample of Chinese was obtained and the basic databases was established; Clinic data about epidemiology of venous thromboembolism which confirmed PTE as a common disease in China also was harvested; this study also found the heredity characteristics of high coagulation in Han nationality and discovered the clinical pathological law of PTE in Chinese people. Also the normative diagnosis and treatment and basic study about PTE was enhanced and correct recognition about PTE in China was formed in the field of Chinese medical science which resulted in the diagnosis case of PTE increased more than tenfold.
    中西医结合专题
    Integration of Chinese and Western Medicine Is Outcome of the Medical Development
    Wang Baoen;Han Yu
    2009, 30(1):  8-10. 
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    Chinese medicine and Western medicine have the different history and theory system, but both of them have the same aim in the improvement of human being’s health. The same aim decides the integrated of Chinese and Western medicine during the respective process of development. There are more than fifty years in the integrated of Chinese and Western medicine in clinic. The special and effective results prove the necessity of the existence of the integrated of Chinese and Western medicine. We should take the advantages in both Chinese medicine and Western medicine, and admit their combination may improve the curative effect in clinical practice.
    Understanding the Shortness of Traditional Chinese Medicine and Western Medicine, Taking the Advantage of Integration of Both Medicine in Brain Disease
    Fan Yongping
    2009, 30(1):  11-14. 
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    The concept of brain disease in traditional Chinese medicine had a long history, and a abundant materials could be found. Brain doesn't belong to five Zang organs, but included in extraordinary Fu-organs in the ancient time, which lead to less recognition of brain. Today, brain diseases can be diagnosed quickly and accurately with the help of modern science and technology, but the curative effect treatment in Western medicine are not satisfied by both doctors and patients. Chinese medicine is taking some advantages in the treatment of following brain diseases, complications post brain operation (such as fever post brain operation), multiple sclerosis, Parkinson's disease, cerebral infarction, and so on. The integration of advantages of both Chinese and Western medicine may largely improve the diagnosis and treatment of brain diseases.
    Effects of Erhuang Capsule on Cell Immunity of Mice with Experimental Autoimmune Encephalomyelitis
    Zhao Hui;Wang Lei;Gong Muxin;Liu Yan;Mu Yang;Wang Yizhou;Li Ming;Qi Fang;Fan Yongping;Gong Haiyang;Wang Yajie
    2009, 30(1):  15-19. 
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    Objective To observe the effects of Erhuang Capsule on cell immunity of mice with experimental autoimmune encephalomyelitis(EAE). Methods EAE models C57BL/6 mice were induced by injecting subcutaneously myelin oligodendrocyte glycoprotein 35-55 peptide in complete Freund's adjuvant(CFA), assisted by injecting intraperitoneally with pertussis toxin (PTX) . The animals were weighted and graded daily for clinical disability. At 12, 25 and 40th day after immunization, lymphocyte subgroups and NK cells in the peripheral blood of mice were detected with flow cytometry. Pathologic identification of the brain tissue were done by HE staining. Results The mice in model group were affected beginning on day 9 and reached the peak on day 22. The CD3+ and CD4+ in the lymphocyte subgroups increased significantly(P<0.01), and the ratio of CD4/CD8 of T lymphocyte subsets elevated in active phase(P<0.05). At the remission stage, the percentage of T lymphocyte subset CD8+ and NK cells declined significantly(P<0.05). In pathology, infiltrated inflammatory cells were observed to form sleeves around the vein and the cerebral neurons showed nucleus pyknosis. After the treatment of Erhuang Capsule, the neurological score declined(P<0.05) and HE staining showed mild inflammatory infiltration. As compared with the model group, the ratio of CD4/CD8 of T lymphocyte subsets was significantly lowered in active phase (P<0.01), while, the levels of T lymphocyte subset(CD3+ and CD8+) and NK cells increased obviously at the remission stage(P<0.05). Conclusion Erhuang Capsule may prevent and cure EAE by regulating the T-lymphocyte subsets(CD3+, CD4+, CD8+ and CD4+/CD8+ ratio) and NK cells.
    Influences of Erhuangfang on Cytokines and Balance of Th1/Th2 in EAE Rats
    Zhou Li;Fan Yongping;Wang Lei;Gong Haiyang
    2009, 30(1):  20-23. 
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    Objective To compare clinical course and the level of Th1/Th2(IFN-γ/IL-4) from blood of lewis rats with experimental autoimmune encephalomyelitis(EAE); and to elucidate the functions of Erhuangfang in regulating autoimmune diseases. Methods Lewis rats were immunized with the myelin basic protein(MBP). They were grouped randomly into normal group, EAE-group, prednisone group and Erhuangfang group post immunization(PI). They were monitored daily after PI, and the clinical scores of the degree of paralysis were recorded. On day of 7, 14 and 28 PI, the peripheral blood were collected for cytokines examination. Results On day of 28 after PI, the level of IL-4 was significantly higher(P<0.01) in Erhuangfang group than in EAE group and prednisone group. On day of 28 after PI, the Th1/Th2 level was significantly lower(P<0.01) in Erhuangfang group than in EAE group and prednisone group. Conclusion Erhuangfang can alleviate the clinical signs, upregulate the IL-4 and downregulate the Th1/Th2 levels of serum in EAE.
    Effect of Jianpibushenfang on Ketamine-induced Memory Impairment in Mice
    Jia Hongxiao;He Shuchang;Tang Yongyi;Jiang Zhenkang;Zhang Jizhi
    2009, 30(1):  24-26. 
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    Objective To investigate the effect of Jianpibushenfang(JPBSF) on Ketamine-induced memory impairment in mice. Methods Memory errors of mice in training and retesting 24 hours later were measured by Step-down method. The memory acquisition impairments were induced by i.p Ketamine 20 and Anisodine 10 minutes before training in Step-down performance, respectively. Glutamate in mice brain was analyzed by automated amino acid assay. Results Memory errors of saline+ketamine group in retesting was more significant than that of saline+saline group(P<0.001), while memory errors of JPBSF low dose+ketamine group, JPBSF large dose+ketamine group and piracetam+ketamine group were significantly milder than that of saline+ketamine group(P<0.01). There was no difference in glutamate content in mice brain between JPBSF low dose+ketamine group and saline+ketamine group(P>0.05). Memory errors of saline+anisodine group in retesting was obviously more than that of saline+saline group(P<0.001), while memory errors of JPBSF low dose+anisodine group, JPBSF large dose+anisodine group and piracetam+ketamine group were significantly milder than that of saline+anisodine group(P<0.01). Conclusion JPBSF has improved ketamine and anisodine-induced memory acquisition impairment, but did not change the glutamate level in mice brain.
    Therapeutic Effect of Qishenhuoxue Granule on Severe Sepsis
    Su Yanli;Wang Hong;Zhang Shuwen;Ren Aimin;Zhang Lixia;Wang Chao;Yin Zhenhong;Wen Yan;Wang Baoen
    2009, 30(1):  27-31. 
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    Objective To study the therapeutic effect of Qishenhuoxue Granule on severe sepsis. Methods The study was a prospective, randomized, multipal centered trail. One hundred and sixty-seven eligible patients with severe sepsis from 5 hospitals were enrolled and randomly divided into two groups: eighty-five in control group and 82 in herbal treatment group. Patients were monitored for changes in laboratory variables including TNF-α, IL-6, IL-10, and D-dimer et al, as well as calculations of Marshall score, APACHE Ⅱ score,the length of staying in ICU and rate of 28-day death. Results In the herbal treatment group, IL-6, TNF-α and D-dimer levels were significantly decreased, the length of staying in ICU was shortened after therapy(P<0.05) and the rate of death from any cause within 28 days after treatment was lower than that of the control group. As for mean scores of both Marshall and APACHE Ⅱ scoring systems, no significant difference was found between them. Conclusion Chinese herbal preparation of Qishenhuoxue Granule could reduce the blood levels of IL-6, TNF-α and D-dimer, shorten the length of ICU staying, and reduce the rate of death from any cause at 28 days, and have an acceptable safety profile.
    Correlation between Clinical Symptoms and Brain Function Change in Unipolar Depression: An fMRI Study
    Li Li;Ding Ni;Xue Feng;Mei Leilei;Dong Qi
    2009, 30(1):  32-36. 
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    Objective To investigate the correlation between brain activation and the clinical symptoms evaluated by Hamilton depression rating scale(HAMD 24 items) in unipolar depression patients. Methods Using emotional face matching task and fMRI, brain function of 12 female unipolar depressed patients was studied. They were also rated on the Hamilton depression rating scale. The correlation between brain function change and HAMD scores was studied. Results In sad condition of the emotional face matching task, brain function change in four regions were found significantly correlated with HAMD scores negatively: Inferior Frontal Orbital Cortex(L) vs. score of cognition impediment factor; Inferior Frontal Orbital Cortex(R) vs. score of cognition impediment factor and score of sleeping factor; Temporal Inferior cortex(L and R) vs. score of somatic symptom factor. Conclusion Unipolar depression patients’ clinical cognition impediment, especially their unexplained various somatic symptoms may relate with function change in certain brain areas.
    Clinical Observation of 65 Cases with Hepatocellular Carcinoma Treated by Huqisan and Transeatheter Arterial Chemoembolization
    Yang Wei;Yang Huasheng;He Xiujuan;Li Xiuhui;Qian Ying
    2009, 30(1):  37-40. 
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    Objective To observe the joint effects of Chinese medicine Huqisan(HQS) and transcatheter arterial chemoembolization(TACE) in patients with hepatocellular carcinoma(HCC) on survival time, quality of life and immune function. Methods Sixty-five patients with HCC were randomly divided into two groups: the treatment group with HQS and TACE, and the control group with Huaier Keli. After the treatment for 24 weeks, the differences in peripheral T-lymphocyte subsets, quality of life(KPS), survival time and clinical efficacy of the overall syndrome were analyzed. Results The treatment group was better than the control group in terms of peripheral blood T-lymphocyte subsets, the quality of life(KPS), survival time and clinical efficacy of the overall syndrome. Conclusion The treatment of HQS and TACE for HCC may improve the overall efficacy and the quality of life, survival time and cell immune function of patients.
    Effect of Qishenhuoxue Granule Combined with Fluconazole on the Immune Function of Mice with Systemic C. albicans Infection
    Li Shirong;Wang Hong;Zhang Shuwen;Wang Baoen
    2009, 30(1):  41-43. 
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    Objective To detect the effect of Qishenhuoxue Granule(QHG) combined with fluconazole on the immune function of mice with systemic C. albicans(CA) infection. Methods Deep CA infection mice model were established by injecting standard strain of CA via caudal vein,and were divided into 4 groups at random, treated by gastrogavage with normal saline(group B), fluconazole(FCZ)+QHG(group C), fluconazole(FCZ groupD), and a blank group(group A) with normal mice for control. The MHC Ⅱ positive rate expressed on monocyte and the content of interleukin 2(IL-2) in blood serum were tested pretherapy and on the first day, the third day, the fifty day and the seventh day after treatment. Results QHG can increase MHC Ⅱ expressed on the monocyte and the level of IL-2 in serum. Conclusion The effect of QHG combined with FCZ is better than that of FCZ only, and one of the possible leven mechanism is to strengthen anti-infection ability of mice by regulating immunologic system.
    鼻生理功能专题
    Clinical Evaluation of Nasal Physiology
    Han Demin;Zhang Luo;
    2009, 30(1):  44-48. 
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    Recently, endoscopic sinus surgery and advances of rhinological research in China have undergone tremendous progress and great achievements. However, contract to current technological advancements the understanding of the pathophysiology in nasal diseases need to be improved. The physiological functions of nose include respiratory function, nasal resistance, olfaction, mucociliary clearance, filtration, air conditioning, immunological function, sensation and voice resonance. And the reliable methods of clinical evaluation are under development.
    Exprimental Study on Foxp3 mRNA Expression in the Peripheral Blood Monocyte of Mice with Allergic Rhinitis
    Xi Lin;Han Demin;Fan Erzhong;Li Ying;Jin Shanzhe;Zhang Wei;Zhang Luo;
    2009, 30(1):  49-53. 
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    Objective To investigate the level of transcription factor Foxp3 rhinitis level in the peripheral blood monocyte(PBMC)of mice with allergic rhinitis, and its relationship with pathogen of allergic AR. Methods 30 female BALB/c mice were divided into 2 groups:ovalbumin(OVA)group and control. After 21 days the AR models were established. The pathological differences between the two groups were then surveyed. Reverse transcription-polymerase chain reaction(RT-PCR)was used to examine Foxp3 mRNA expression in the spleen PBMC from the two groups. Results In contrast to the control, OVA models had significant pathological changes in the nasal cavity, including epithelial damage and eosinophil infiltration in the submucosa. Foxp3 mRNA expression was lower in subjects with AR than that in the controls(P<0.05). Conclusion The expression of Foxp3 mRNA in the OVA group is low which suggests that Foxp3 may play an important regulatory role in the pathogenesis of AR.
    Primary Studies on Phosphorylation Profile of Ciliary Proteins in Trachea of Rabbit
    Liu Chen;Han Demin;Zhang Luo;
    2009, 30(1):  54-56. 
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    Objective To establish a method of cilia isolation from airway epithelium and provide the methods of further studying the role of the protein phosphorylation in the mechanisms of ciliary motility. Methods The rabbit trachea epithelium was incubated in isolation buffer containing Triton X-100 in order to isolate the cilia from the epithelial tissue. Cilia were then collected by centrifugation. The sample was examined by electron microscopy, and the β-tubulin and the phosphorylated proteins were visualized by Western blotting. Results 1 The electron micrograph demonstrated a highly enriched ciliary preparation showing the characteristic features of intact axoneme; 2 Western blotting analysis showed visible bands of β-tubulin; 3 Western blotting analysis detected five tyrosine phosphorylated bands. Conclusion The ciliary preparation can be obtained by incubating the rabbit airway epithelium in isolation buffer containing Triton X-100 and the following centrifugation. The preparation can be used to study the role of the protein phosphorylation in the mechanisms of ciliary motility.
    Effect of ATP on Human Nasal Ciliary Beat Frequency
    Shi Linggai;Han Demin;Zhang Luo;
    2009, 30(1):  57-61. 
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    Objective To investigate the effects of ATP on human nasal cilia movement. Methods Ciliary beat frequency(CBF) of cultured human nasal epithelial cells was measured by high-speed digital microscopy in ATP solution of different concentrations. Results CBF of cultured nasal epithelial cells exposed to HBSS showed no significant changes in 3 minutes(P>0.05); However in 1, 10 and 100 μmol/L ATP solution, CBF increased significantly in 10 seconds and reached the apex, then it showed a sustained elevation in 3 minutes. In 1 μmol/L ATP solution, CBF increased significantly from(9.2±1.8)Hz to(10.3±2.0)Hz(n=10)(P<0.05). Similarly in 10 μmol/L ATP and 100 μmol/L ATP solution, CBF increased significantly when compared with samples in HBSS. Conclusion When 1, 10 and 100 μmol/L ATP were added to the chamber, there were rapid increase in ciliary activity. In addition, there is concentration-dependent relation between CBF and ATP.
    Effects of Myrtol Standardized on Human Nasal Mucociliary Transport
    Wang Nan;Han Demin;Song Xiaohong;Zhang Wei;Zhang Luo;
    2009, 30(1):  62-65. 
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    Objective To investigate the effects of Myrtol Standardized on the regulation of healthy human nasal ciliary beat frequency(CBF) and its influence on nasal mucociliary transport time(MTT). Methods Changes in human nasal CBF in response to increasing concentrations of Myrtol Standardized within 20 minutes were quantified by using of high-speed digital microscopy. The MTTs before and after application of Myrtol Standardized in 12 patients with chronic rhinitis were determined by using the saccharin test. 9 patients were examined nasal function test. Results No statistically significant difference was identified when compared to basal CBF at the concentration of 75 μg/L, 150 μg/L or 300 μg/L, Myrtol Standardized. In addition, the use of Myrtol Standardized decreased the mean human nasal MTT from(12.8±4.5) to (9.3±3.8)min (n=12). Conclusion Myrtol Standardized significantly increased MTT in vivo, but no obvious effect on human nasal CBF in vitro was observed.
    Evaluation of Nasal Patency of Patients with Nasal Septum Deviation
    Cao Chunting;Han Demin;Zhang Luo;
    2009, 30(1):  66-69. 
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    Objective To find out the correlation between the measurement of four-phase rhinomanometry and subjective symptom of nasal obstruction, and the relation between nasal septum deviation(NSD)and the measurement result of the four-phase rhinomanometry as well as the acoustic rhinometry. Methods Group1 of subjects: patient suffering from nasal obstruction caused by the septum deviation: including 25 male nasal cavities and 5 female nasal cavities. Group2: normal subjects including 14 men(28 nasal cavities) and 3 women(6 nasal cavities). Group3: Asymptomatic subjects with septum deviation including 21 men and 4 women cavities. HRR2 four-phase rhinomanometer and eccovision acoustic rhinometer were used to acquire the the effective resistances and vertex resistance in inspiration and expiration process(Reffin, Reffex, VRin, VRex), nasal cavity volume of 0~1 cm, 1~2 cm, 2~5 cm, 5~7 cm from nostril(V1, V1-2, V2-5 and V5-7), minimum cross-sectional area(MCA ) and the distance between the nostril to minimum cross-sectional area( MD)of each nasal cavity. Results The VAS of subjective obstruction symptom has no significant correlation to the results of the four-phase rhinomanometry and the acoustic rhinometry except V2-5. In Group1, the Reffin, Reffex, VRin and VRex are significantly correlated with V1-2, V2-5, V5-7 and MCA except V1. For the Reffin, Reffex, VRin and VRex, the difference between Group1 and Group 2, and Group1 and Group3 were statistically significant, however, no statistic significance between Group2 and Group3 were found. There was no significant statistic difference in V1, V1-2 and MCA between Group 1 and Group2, and Group1 and Group3, however, there were significant statistic difference in V2-5, V5-7 and MD. There was no significant statistic difference in V1, V1-2, V2-5, V5-7, MCA and MD between Group2 and Group3. Moreover, the MD of Group 1 apparently backward shifts comparing with the other two Groups, near the 2 cm from nostril. The measurement results by the four-phase rhinomanometry and the acoustic rhinometry on the NSD patient with the nasal obstruction symptom have a significant correlation. The combination of the two methods can reflect the influence from the geometrical variance of the nasal cavity on the nasal airflow. Conclusion The Reffin, Reffex, VRin and VRex of the pure NSD patient with the nasal obstruction symptom are significant higher than the normal group and the asymptomatic NSD group.
    Progress of Study on Cilia
    Jiao Jian;Zhang Luo;
    2009, 30(1):  70-75. 
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    Cilia are located on almost all cell types of the human body, the basic structure includes a microtubule cytoskeleton, the axoneme, and the surrounding ciliary membrane. Cilia perform key tasks for a variety of organs, but defects in the structure or function of them are involved in the pathogenesis of several cilia-related disorders. Mammalian cilia occur in two major pattern: motile 9+2 cilia and usually nonmotile 9+0 primary cilia, which function as a sort of cellular antenna, to pick up signals from the environment and pass them on to the cell's nucleus. Mammalian ciliary beating is regulated by cAMP, cGMP, PKC as well as Ca2+ and intracellular pH. To further explore the structure and function of cilia, and the regulation mechanism of mammalian ciliary beat frequency is of great importance for the diagnosis and treatment of certain cilia-related disorders.
    基础研究
    Effect of MPTP on the mPer1 mRNA Expression in the Brain of C57BL/6J
    Zuo Xiaohong;Cai Yanning;Yu Shun;Liu Shu;Chen Biao
    2009, 30(1):  76-79. 
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    Objective To study the mPer1 mRNA expression profiles in striatum and other brain regions in MPTP-treated mice. Methods Sixty C57 BL/6J male mice were randomized into MPTP and control groups. For MPTP group, MPTP-HCL was administered intraperitoneally in 5 consecutive days. For the control group, MPTP-HCL was replaced by same volume of saline. On the 11th day, mice were sacrificed at six different time points during a 24-hour periods, and striatum and the rest of brain regions were isolated. Dopamine levels of striatum were measured using HPLC-ECD. The mRNA expression of mPer1 was examined using real time PCR. Results The dopamine levels in striatum decreased about 80% in MPTP-treated mice, which remained at low level for up to 10 days. The expression of mPer1 mRNA in striatum increased significantly in MPTP-treated mice, with the rhythmic expression phase being shifted. Compared with control mice, the expression peak reached 4 hours earlier. The mPer1 mRNA expression in the rest of brain regions was also increased significantly in MPTP-treated mice, but no rhythmic changes occurred. Conclusion MPTP can change the mRNA expression of mPer1 in the striatum and the rest of brain regions, indicating that MPTP may affect the intracellular circadian oscillator in the brain.
    Effects of Mistletoe Alkali and Mistletoe Polysaccharose Proliferation and Apoptosis of Hepato Carcinoma Cells
    Dong Kun;Feng Ping;Jiang Ying;Fan Xing;Li Xiangli;Xue Xiaowei;Wang Xuejiang
    2009, 30(1):  80-84. 
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    Objective To investigate the effects of Mistletoe Alkali(MA) and Mistletoe Polysaccharose(MP) on proliferation and apoptosis in hepatoma carcinoma cells. Methods In vitro experiments with mistletoe polysaccharides, and mistletoe alkali induced in hibition of SMMC-7721 and HepG2 proliferation were observed by MTT assay. The cell cycle, the rate of apoptosis and the DNA relative amount of SMMC-7721 and Hep G2 were examined by flow cytometry. The migrating changes of hepato carcinoma cell generation cycle were analyzed at every period. Results The results of in vitro experiments showed that both MA and MP could inhibit the proliferation of SMMC-7721 and Hep G2 cells, and promote their apoptosis in a time and dose-dependent manner. After treatment with the drugs, the cell population in the groups of MP and MA increased at G1 phase(81.0%, 86.9% vs 70.0%, P<0.01 ) and decreased at G2 phase and S phase(13.1%, 5.7%vs 16.4%, P<0.01; 5.9%, 7.4% vs 13.5%, P<0.01), in comparison with those in the controls. In addition, both of the two drugs enhanced the apoptosis of SMMC-7721 and Hep G2 cells(MP: 12.3%,11.7% vs 6.0 %, P<0.01; MA: 7.5%,6.7% vs 6.0%, P<0.01). Conclusion MA and MP could inhibit the proliferation and promote the apoptosis of SMMC-7721 and Hep G2 cells.
    Clinical Significance of CD34 and CK34βE12 Expressions in Lesions of the Cervix
    Zhang Hongkai;Du Qiang;Yan Meishen;Zhao Tiehua;Yan Lingling;Chen Weidong
    2009, 30(1):  85-88. 
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    Objective To study the CD34 and CK34βE12 expressions in the squamous intraepithelial lesions of the cervix and their clinical significance. Methods The S-P immunohistochemical method to detect CD34 and CK34βE12 expressions was applied to in 120 samples. Results The positive rate of CD34 and CK34βE12 in the inflammatory lesions were 70% and 86%, respectively; and in CIN Ⅰ the positive rate of CD34 was 71%, and the positive rate of CK34βE12 was 81%;in CIN Ⅱ the positive rate of CD34 was 77%, the positive rate of CK34βE12 was 66%; in CIN Ⅲ the positive rate of CD34 was 97%, while that of CK34βE12 could reach 100%. In addition, the expression pattern of the two proteins were related to the grade of cervical lesions(P<0.05). Conclusion The expressions of CD34 and CK34βE12 were correlated with CIN grading and these the two protein expressions may provide certain clinical significance in the diagnosis of CINs in the cervix, but not the canncer type.
    临床研究
    Role of C-reactive Protein in the Relationship between Obstructive Sleep Apnea-hypopnea Syndrome and Coronary Heart Disease
    Fan Xin;Du Fenghe
    2009, 30(1):  89-92. 
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    Objective To explore the role of C-reactive protein in the relationship between obstructive sleep apnea-hypopnea syndrome(OSAHS) and coronary heart disease(CHD). Methods The levels of serum C-reactive protein(CRP) in 31 OSAHS patients with CHD, 31 OSAHS patients without CHD and 28 healthy controls was examined, and the relationship of the CRP concentration with the polysomnogram parameters was analyzed. Results The levels of serum CRP in OSAHS patients with CHD and OSAHS patients without CHD were significantly higher compared with the healthy controls(P<0.05). The level of CRP was also significantly higher in OSAHS patients with CHD than that in OSAHS patients without CHD(P<0.05). Conclusion The high level of serum CRP might be an important risk factor for the development of CHD in OSAHS patients.
    Determination of HER-2/neu Gene Expression in the Tissue of Breast Cancer by FISH and Its Clinicopathological Implications
    Yu Wei;Gu Junchao;Xu Xiuhong;Song Maomin;Bai Qingyang;Liu Jianzhong
    2009, 30(1):  93-96. 
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    Objective To explore overexpression of HER-2/neu gene by fluorescence in situ hybridization(FISH) in breast cancer and explore the clinical implications. Methods HER-2/neu gene in 33 cases with kainogeneous female breast cancer was detected by FISH from January 2008 up to now, and the relationship between HER-2/neu gene overexpression with clinical and pathological characters investigated. Results HER-2/neu gene expression rate was 39.4%. The overexpression was not correlated with tumor size, menostasis and clinical tumor stage(P>0.05). It was positively correlated with axillary lymph node status(P<0.05) and P53 gene(P<0.05); and negatively correlated with ER(P<0.01), but not correlated with PR(P>0.05). The overexpression cases with HER-2/neu gene showed 11 strongly and 5 moderately positive expression of HER-2 protein by immunohistochemistry. Conclusion FISH technique could detect the overexpression of HER-2/neu gene in breast cancer. It has favourable concordance with strongly positive expression of HER-2 protein(3+), but in the of moderately positive cases, FISH must be applied.
    Clinical Relevance of Urinary Epithelial Cells Adhesive with Bacteria for Diagnosis of UTI in Old Women
    Bo Yuhong;Jiang Feng;Bi Zengqi
    2009, 30(1):  97-99. 
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    Objective To explore a simple and accurate method for diagnose and treatment of repeated urinary tract infection(UTI) in old female patients. Methods Fifty-five old female patients with repeated UTI were studied. Three methods including phase contrast microscopy examination, routive urine test and urine culture were used to identity if there were some correlations among them. And the specificity and sensitivity of each method were studied. Results The number of epithelial cells adhesive with bacteria significantly correlated with the number of bacteria in urine culture. But the number of urinary white blood cells did not significantly correlate with urine culture results. The sensitivity of the urinary sediment examination by phase contrast microscopy was about 84.8%, and the specificity of the urine routing test was lower, about 51.9%. Conclusion Finding the epithelial cells adhesive with bacteria by phase contrast microscopy examination might be a simple, specific and sensitive method for diagnose UTI.
    Clinical Significance of Platelet Membrane Glycoproteins in Different Period of Ischemic Cerebrovascular Diseases
    Sun Lin;Zhang Xueqin;Wang Yang;Yu Huafeng
    2009, 30(1):  100-103. 
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    Objective To investigate the clinical significance of the platelet membrane glycoproteins in various periods of ischemic cerebrovascular diseases. Methods Peripheral blood platelet activation markers GPⅢa(CD61), GPⅠb(CD42b) and GPⅠa(CD49b) in 108 patients with ischemic cerebrovascular diseases(20 TIAs, 49 lacunar infarcts and 39 cerebral thrombosis) were detected in various periods(acute stage≤3 days, one week, two weeks and convalescence stage) by the method of quantitative flow cytometry. Thirty normal aged persons served as controls. The degree of neurologic impairment were analyzed by ESS grading. Results GPⅢa expression increased significantly(P<0.05) within one week of TIA and in acute stage of both cerebral thrombosis. In convalescence stage, the GPⅠa expression in cerebral thrombosis was significantly higher than those of in lacunar infarcts. Peripheral blood GPⅠa slightly stepped up in acute stage but decreased in convalescence stage(P>0.05). ESS grading was accociated with GPⅠb(pearson analysis, r=0.233, P<0.05) and these GPs were associated with one another by partial correlation analysis. Conclusion GPⅢa and GPⅠb were significantly activated in the acute phase of cerebral thrombosis and TIAs, which was very important in the course of thrombosis. The changes in GP may reflect a prothrombotic state and they may be predictive factors to the progress and prognosis of some ischemic cerebrovascular diseases.
    Prevention and Handling Strategies on Common Complications of Subclavian Central Venous Catheters in Patients with Tumor
    Liu Suqin
    2009, 30(1):  104-106. 
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    Objective To explore prevention and handling strategies on common complications of subclavian central venous catheters (CVC) in patients with tumor. Methods One thousand four hundred and eighty-five patients with malignant tumor undergone subclavian CVC in our hospital from August 2001 to July 2008 were retrospectively analyzed to study correlative complications and handling strategies. Results Complications occurred in 98 cases (6.59 %), including 22 cases of puncturing the artery by mistake(1.48%), four cases of puncturing the internal jugular vein by mistake(0.27%), two cases of pneumothorax(0.13%), three cases of arrhythmia(0.20%), one case of local hematoma(0.06%), thirty-six cases of infection after catheterization(2.42%), twenty-five cases of catheter obstruction(1.68%), five cases of catheter prolapse(0.34%). Conclusion Although subclavian CVC has certain risk, the complications can be prevented and reduced along with more accumulated clinical experience. Subclavian CVC is a safe method to carry out venipuncture for tumor patients.
    Comperative Studies on Postoperative Epidural Analgesic Effects between Morphine and Sulfentanil after Cesarean Section
    Yue Jianning
    2009, 30(1):  107-109. 
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    Objective To evaluate the postoperative analgesic effects of sulfentanil and morphine at loading doses in patients with cesarean. Methods One hundred and twenty ASA I or II women following cesarean section, were randomly assigned to two groups(n=60 each) according to different epidural loading doses: sulfentanil 20 μg and morphine 3 mg group, then contiuous epidural infusion were given with infusion pumps as follows: 0.6 mg/L sulfentanil, 0.15% ropivacaine and 25 mg/L droperidol at infusion rate 2 mL/h. Visual analog scale(VAS), side effects and oral analgesics administration were recorded. Results There was no significant difference in pain from incision and from uterine contraction in both groups at 2~6 h and 18~24 h postoperatively(P>0.05). Pain score and analgesic requirements were higher in sulfentanil group at 7~10 h(P<0.01). Uterine contraction pain was more severe than incisal pain at 2~6 h and 7~10 h postoperatively in both groups(P<0.01). There was no significant difference in pain from incision and from uterine contraction in morphine group, while incisal pain was more severe in sulfentanil group at 18~24 h. There was more satisfaction of pain relief in morphine group(P<0.01), but more side effects of nausea, vomiting and pruritus were recorded(P<0.01). Conclusion Except the side effects, the postoperative epidural analgesic effect of morphine is better than that of sulfentanil, and both have better analgesic effect in incisal pain than uterine contraction pain.
    Studies on the Appropriate Obstetric Managment of Secondary Pregnancy after Cesarean Section
    Yang Yue;Huang Xinghua
    2009, 30(1):  110-113. 
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    Objective To investigate the appropriate obstetric managment of secondary pregnancy after cesarean section. Methods A total of 237 cases with secondary pregnancy after cesarean section from 2005 to 2008 were retrospectively studied. The relevant circumstances of two forms that made up two categories in each form selective re-cesarean section and emergency re-cesarean section; re-cesarean section and vaginal labor had been cautiously compared. Results In 237 labors of secondary pregnancy after cesarean section, there were 158 cases of attempted vaginal labor and 121 cases of vaginal delivery. Hence, the successful rate of attempted vaginal labor was 76.6%(121/158). There were 79 cases with selective re-cesarean section. In emergency re-cesarean section, 31 cases had unrestrictive indication, while was 39.2%(31/79). Consequently it can be said that selective re-cesarean section is strongly related to the psychological elements of the patients and their families. Comparing with volume of delivery blood loss, operation time, dehiscence of uterus incision during operation, postpartum infection, rate of puerperalism and neonatal apnea, there was no significant difference between selective re-cesarean section and emergency re-cesarean section. However, volume of delivery blood loss, postpartum hemorrhage, postpartum infection, rate of puerperalism and days of hospitalization between re-cesarean section and vaginal labor showed significant differences(P<0.05). Conversely, there was no significant difference of neonatal apnea in these two categories(P>0.05). Conclusion It is very important to choose an appropriate labor managment of secondary pregnancy after cesarean section, including meticulous prenatal checkups, good doctor-patient communications, strict handing of restrictive re-cesarean section indications scientifically choosing labor managment and proficient obstetric skills. Attempted vaginal labor in secondary pregnancy after cesarean section is more profitable. Labor trials would not add difficulties and risks in re-cesarean section. The operative skills of primary cesarean section directly affect healing of uterus incision and directly relate to the safety of re-cesarean section. It is not essential to have more than two years apart from the previous cesarean section.
    Report of Reproductive Health Situation among Adolescent in Beijing Area
    Li Hui;Han Lihui;Wu Minghui
    2009, 30(1):  114-118. 
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    Objective To realize the existing problems in reproductive health among adolescent in Beijing area and provide resolvent method. Methods Using questionnaire to study the reproductive health of students in professional high school and university in Xicheng and Haidian districts. The questionnaire included basic situation, understanding and popularization of reproductive health, sexual activity, and service of reproductive health. Results 1 Among different sexual distinction, the proportion of having sexual activity was different. In female student, the proportion is obviously higher than in males(P<0.01). Among the students that having sexual activity, 11.9% had pregnancy and abortion history. There is remarkable difference between males and females(P=0.022).14% had symptoms of genital tract infection; 74.6% had contraceptive measure, and 78.3% of these student using condom; 71.4% of male and 63.8% of female know emergency contraception. 2 As the social characteristic, the profession of 60.7% father and 50% mother were white collar. 3 21.7% students select professional workers to consult. 53.2% male used internet and 72.5% female used medium to get contraception and reproductive health information(P<0.01). There is also remarkable difference between males and females in the the transmitting route of HIV.78.6% male and 75.8% female knew condom could prevent HIV. Conclusion 1 It should be highly concerned that the proportion of having sexual activity is male among investigated addescents. 2 25.3% of students who have more than one sexual partner and are risk group of HIV and genital tract infection. It is important to strengthen the education and protection for these students. 3 The students whose parents having high salary tend to have sexual activity and should be educated. 4 Condom is common and feasible to receive, The popularization and delivery work should be carried out, meanwhile, knowledge of emergency contraception should be provided to protect the health of addescents. 5 Sexual experience popularization should be strengthen through internet and medium according to the different characters of male and females. 6 In order to provide more professional and suitable service for adolescents, professional service and consultant work should be performed well and efficiently.