Expression of Splicing Factor SF2/ASF in Childhood Leukemia Cell

doi:10.3785/j.issn.1006-7795.2009.03.003

Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (3): 273-276.doi: 10.3785/j.issn.1006-7795.2009.03.003

Expression of Splicing Factor SF2/ASF in Childhood Leukemia Cell

GAO Hui-fang^1,2, ZHANG Han^1,2, PEI Pei³, LIU Yi^1,2, LI Zhi-gang^1,2, JIANG Jin^1,2, ZHANG Rui-dong^1,2, GAO Chao^1,2, QI Yu³, ZHENG Hu-yong^1,2

1. Hematology Center, Beijing Children's Hospital, Capital Medical University;2. Department of Oncology, Capital Medical University;3. Department of Pediatrics, First Hospital, Peking University

Received:2009-03-18 Revised:1900-01-01 Online:2009-06-21 Published:2009-06-21

Abstract

Abstract:

Objective To investigate the expression of splicing factor SF2/ASF in pediatric acute B-lymphoblastic leukemia(B-ALL) cells and in leukemia cell lines treated with chemotherapeutic agents. Methods The protein levels of SF2/ASF were measured in bone marrow samples during preliminary diagnosis and complete remission(CR) B-ALL children by Western blotting; the protein levels of SF2/ASF, before and after vincristine and cytarabine treatment of B-ALL cell line of NALM-6, were also detected by Western blotting. Results Western blotting showed there was a strong SF2/ASF expression in children with preliminarily diagnosed B-ALL children compared with that in children who had complete remission and in children of the control group composed of idiopathic thrombocytopenic purpura(ITP) cases; high expression of SF2/ASF was detected in NALM-6 cell lines, but the expression reduced after treatment with cytarabine and vincristine. Conclusion Splicing factor SF2/ASF was expressed significantly higher in bone marrow of B-ALL children and leukemia cells, but was down-regulated in CR patients and in B-ALL cells treated with cytarabine and vincristine, indicating that SF2/ASF is a practical marker for monitoring outcome of B-ALL treatment.

Key words: leukemia, splicing factor, SF2/ASF, vincristine, cytarabine

CLC Number:

GAO Hui-fang;ZHANG Han;PEI Pei;LIU Yi;LI Zhi-gang;JIANG Jin;ZHANG Rui-dong;GAO Chao;QI Yu;ZHENG Hu-yong;. Expression of Splicing Factor SF2/ASF in Childhood Leukemia Cell[J]. Journal of Capital Medical University, 2009, 30(3): 273-276.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3785/j.issn.1006-7795.2009.03.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2009/V30/I3/273

[1]	Zhu Ping, Yu Huan, Fu Limei, Liao Xin, Xiong Mujun, Lei Aming, Zhu Pingan, Liang Xinquan. Clinical analysis of umbilical cord blood transplantation in the treatment of refractory and recurrent acute leukemia [J]. Journal of Capital Medical University, 2021, 42(6): 1041-1045.
[2]	Jia Chenguang, Zhou Xuan, Wang Bin, Zhu Guanghua, Qin Maoquan. Study of allogeneic stem cell transplantation for pediatric acute myeloid leukemia [J]. Journal of Capital Medical University, 2019, 40(2): 163-168.
[3]	Zhang Liqiang, Chen Zhenping, Zheng Jie, Ma Jie, Wu Runhui. A evaluation of the early efficacy and safety of generic imatinib in treating children of chronic myeloid leukemia in chronic phase [J]. Journal of Capital Medical University, 2019, 40(2): 174-178.
[4]	Peng Xiaojuan, Zhang Ruijuan, Wang Meifang, Zhang Yaofang, Tan Yanhong, Yang Linhua. Clinical efficacy of decitabine sequential or simultaneous with “3+7” regimen for acute myeloid leukemia [J]. Journal of Capital Medical University, 2019, 40(2): 300-304.
[5]	Ma Xiaowen, Dong Chunxia, Chen Jianfang, Wang Meifang, Zhang Ruijuan, Zhang Jianhua, Yang Linhua. Prognostic value of absolute monocyte count in chronic lymphocytic leukemia [J]. Journal of Capital Medical University, 2018, 39(4): 581-585.
[6]	Qin Na, Li Guangbo, Guo shuxia, Zhang Xiaojuan. Expression of microRNA-221 in chronic myeloid leukemia and its mechanism [J]. Journal of Capital Medical University, 2017, 38(4): 580-585.
[7]	Duan Yanlong, Jin Ling, Yang Jing, Huang Shuang, Zhang Meng, Lai Rui, Zhou Chunju, Zhang Yonghong. Clinical characteristics of 26 children with Burkitt's leukemia and outcome of treatment with revised LMB 89 protocol [J]. Journal of Capital Medical University, 2016, 37(2): 141-147.
[8]	Wu Xuebin. Chronic neutrophilic leukemia [J]. Journal of Capital Medical University, 2014, 35(5): 577-580.
[9]	Wang Min, Wang Liru, Cui Jianying, Zhou Hebing. Expression of TGF-β₁ in hyperleukocytic acute myeloid leukemia and its clinical significance [J]. Journal of Capital Medical University, 2014, 35(5): 555-559.
[10]	Meng Guangqiang, Wang Liru, Chen Yijuan, Zhang Jingjing, Wang Min, Guo Huixia, Wu Yue. Clinical application of leukapheresis in patients with hyperleukocytic acute leukemia [J]. Journal of Capital Medical University, 2014, 35(5): 566-571.
[11]	ZHAO Haiping, LUO Yumin. Progress in studies of MicroRNA-144-associated diseases and related mechanism [J]. Journal of Capital Medical University, 2013, 34(1): 80-85.
[12]	Wang Hongyan;Chang Ying. rsTRAIL-induced Apoptosis of Acute Leukemic Cells and Its Synergy with Chemotherapeutic Drug [J]. Journal of Capital Medical University, 2008, 29(3): 364-368.
[13]	Yang Lei;Liu Fuqiang;Wang Jingwen;Wu Yiping;Ding Jing. Study of the Effects of Specific CTL Induced by DCs Treated with Sodium Selenite and ACL on Leukemia Cell line K562 [J]. Journal of Capital Medical University, 2008, 29(2): 137-141.
[14]	Ji Bingxin;Su Li;Liu Congyan;Zhao Hong;Zhang Chenwei;Hui Wuhan;Xu Juan;Tian Ding. Analysis of Autologous Hemopoietic Stem Cell Transplantation for Hematological Malignancies [J]. Journal of Capital Medical University, 2008, 29(2): 142-145.
[15]	Li Yaohua;Xu Juan;Sun Xuejing;Liu Congyan;Wan Suigui. CD7 Expression and its Significance in the Detection of Minimal Residual Disease in Adult Acute Myelocytic Leukemia [J]. Journal of Capital Medical University, 2006, 27(2): 233-236.

Expression of Splicing Factor SF2/ASF in Childhood Leukemia Cell

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments