Abstract: Objective To investigate the effect of insulin sensitizer rosiglitazone on streptozotocin(STZ)induced insulin signal transduction pathway injury in SH-SY5Y cells. Methods Cells from human neuroblastoma cell line SH-SY5Y were divided into control group, STZ 0.8 mmol/L group, STZ 0.8 mmol/L+rosiglitazone 20 μmol/L group. The cells were counted, MTT(MTT) metabolic rate, lactate dehydrogenase(LDH) leakage rate in each group were measured, and the effect of rosiglitazone on SH-SY5Y cells was observed. Results Compared with the control group, cell count and MTT metabolic rate of STZ model decreased(P<0.01), and LDH leakage rate increased(P<0.01). After rosiglitazone protection, these indicators were significantly improved, cell count and MTT metabolic rate were higher than that in STZ injury group(P<0.01), LDH leakage rate was lower(P<0.01). Conclusion Rosiglitazone can reduce STZ-induced nerve cell toxicity, to improve cell survival, though its mechanism still need to be further explored.

Key words: human neuroblastoma cell line SH-SY5Y, streptozotocin, rosiglitazone