Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (3): 388-392.

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Molecular Typing of Salmonella Paratyphi A Strains from Guiyang of Guizhou Province with Pulsed Field Gel Electrophoresis

YAO Guang-hai1,2, WANG Tao1*, DIAO Bao-Wei3, ZHOU Hai-jian3, TIAN Ke-cheng2, YOU Lv2, WEI Xiao-yu2, TANG Guang-peng2, WANG Ding-ming2, KAN Biao3   

  1. 1. Department of Epidemiology and Biostatistics, School of Public Health, Peking University;2. Department of Epidemiology, Institute of Communicable Disease Control and Prevention, Center for Disease Control and Prevention, Guizhou Provine; 3. State Key Laboratory of Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-21 Published:2010-06-21
  • Contact: WANG Tao

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Abstract:

Objective To type and group the Salmonella paratyphi A strains isolated in Guiyang from 2000 to 2007 and to understand the epidemiological features caused by the pathogen. Methods Pulsed field gel electrophoresis(PFGE) of large restriction fragments of bacterial chromosomal DNA was applied to type the Salmonella paratyphi A strains. Results Ten PFGE patterns were identified among the 146 S. paratyphi A isolates, where 32 isolates belonged to pattern 1(21.9%), 73 isolates to pattern 2(50.0%), 13 isolates to pattern 3(8.9%), 7 isolates to pattern 4(4.8%), 14 isolates to pattern 5(9.6%), 3 isolates to pattern 10(2.7%) and each of the remaining 3 isolates to an independent pattern(0.7% respectively). The PFGE type of strains varied year by year, patterns 1 and 2 was domain epidemic patterns; 24 strains from 2 outbreaks had 5 PFGE patterns and the proportion of pattern 1 was 54.2% and the strain isolated from a healthy carrier belonged to pattern 1. Strains isolated from high epidemic areas had 9 PFGE patterns based on the size and number of restriction fragments and patterns which were digested by Spe I. Conclusion The findings of the present study verified the usefulness of PFGE in characterizing and comparing strains of Salmonella Paratyphi A, and forecasting outbreak. The results indicated that although the distribution of PFGE patterns varied among different counties of Guiyang，the pattern 1 and pattern 2 were the epidemic domain, which were responsible for paratyphoid fever in high epidemic counties.

Key words: salmonella paratyphi A, molecular typing, pulsed field gel electrophoresis

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