Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (5): 610-613.

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Expression of Sphingosine 1 phosphate Receptors in Fibrotic Liver Induced by Carbon Tetrachloride

YANG Lin1,2, ZHAO Dong-xu1*, LI Li-ying2*   

  1. 1. Department of Bioengineering School of Life, Beijing Institute of Technology; 2. Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-21 Published:2010-10-21
  • Contact: ZHAO Dong-xu，LI Li-ying

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Abstract:

Objective To determine the mRNA and protein expression of sphingosine 1-phosphate receptors 1, 2 and 3(S1P1-3) in the normal and fibrotic liver. Methods ICR mice were randomized into two experimental groups, control [olive oil(OO) treated] and model(CCl4 treated) groups. Mice were sacrificed at two or four weeks of treatment. Liver tissue was examined by real-time RT-PCR and Western blotting to analyze the expression of S1P1-3. Results Two or four weeks treatment by CCl4, S1P1 mRNA expression was not significantly different; S1P2, S1P3 mRNA expression was up-regulated(P<0.05) in liver tissue. Correspondingly, S1P1 protein level was not significantly different; S1P2, S1P3 protein level was up-regulated(P<0.05). Conclusion During the process of liver fibrosis, sphingosine 1-phosphate receptor1-3 mRNA expression and protein level were various, the relative mRNA and protein level expression of S1P2, S1P3 have shown increases. Our results indicate that S1P receptors have an extremely important role in liver fibrogenesis.

Key words: liver fibrosis, sphingosine 1phosphate, sphingosine 1phosphate receptor

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