Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (6): 771-776.

• 基础研究 • Previous Articles     Next Articles

Effect of Peanut oil on Expression of Insulin Receptor and Insulin Receptor Substrates in Hippocampal CA1 Area of Rats with Type 2 Diabetes Mellitus Rats

ZHAO Yong-mei1, XU Qiu-yan1, 2, ZHAO Zhi-wei1, JI Zhi-juan1   

  1. 1. Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital, Capital Medical University;2. Department of Ophthalmology, Hospital of China University of Mining & Technology, Beijing
  • Received:2010-05-20 Revised:1900-01-01 Online:2010-12-24 Published:2010-12-24

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Abstract: Objective To study the difference in expression of insulin signal transduction proteins insulin receptor(InsR),insulin receptor substrate-1(IRS-1) and insulin receptor substrate-2(IRS-2) in hippocampal CA1 area of rats with type 2 diabetes mellitus and to investigate the protective effects of peanut oil against diabetic encephalopathy.Methods Sixty healthy male SD rats were assigned into 4 groups randomly:normal control group(C group);type 2 diabetes mellitus group(T2DM group);type 2 diabetes mellitus treated with 2 mL peanut oil group(T2DM+2 mL group) and type 2 diabetes mellitus treated with 5 mL peanut oil group(T2DM+5 mL group).The rats in group C were fed with normal food.Type 2 diabetes mellitus was induced by low dose streptozotocin intraperitoneal injection(25 mg/kg body weight) after feeding with high-fat feed for 2 months.All the rats were sacrificed one month after type 2 diabetes mellitus was induced.The expression of InsR,IRS-1 and IRS-2 in hippocampal CA1 area of rats was studied by immunohistochemical staining.Results The expression of InsR in hippocampal CA1 area of T2DM group decreased significantly compared with that of group C(P<0.05).The expression of InsR in hippocampal CA1 area of T2DM+5 mL group increased significantly compared with that of T2DM group(P<0.05).② The expression of IRS-1 in hippocampal CA1 area of T2DM group decreased significantly compared with that of group C(P<0.05).The expression of IRS-1 in hippocampal CA1 area of T2DM+2 mL group and T2DM+5 mL group increased significantly compared with that of T2DM group(P<0.05).③ The expression of IRS-2 in hippocampal CA1 area of T2DM group decreased significantly compared with that of group C(P<0.05).The expression of IRS-2 in hippocampal CA1 area of T2DM+5 mL group increased significantly compared with that of T2DM group(P<0.05).Conclusion Insulin signal transduction is dysregulated in the hippocampal CA1 area of the type 2 diabetes mellitus rats.This might be one of the reasons that cause diabetic encephalopathy.Peanut oil improves the expression of insulin signal transduction proteins in hippocampal CA1 area of T2DM rats,suggesting that peanut oil might have protective effect against diabetic encephalopathy.

Key words: type 2 diabetic mellitus, insulin receptor, insulin receptor substrate-1, insulin receptor substrate-2, peanut oil

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