Abstract: It is a difficult to carry biological tissue regeneration and functional reconstruction for oral and maxillofacial deformities, especially for dental tissue defects. This project was designed to regenerate tooth , repair the defects of facial bones and reconstruct the function of salivary glands that providing the support for oral cavity. By using gene transfer and salivary gland endoscopy, the new techniques were developed for diagnosis and treatment of obstructive salivary gland diseases and for recovery of irradiation-induced salivary gland dysfunction. We proposed a new concept called as “biological roots” by using of stem cells and tissue engineering technology, the biological roots with mastication function was successfully regenerated in the swine; based on tooth development strategy, new ways for whole tooth regeneration were established and the new seed cells was discovered. Key technologies, parameters and new devices were obtained for development of jaw osteodistraction to improve the complex maxillofacial defects effectively by endogenous jaw bone regeneration. This research and its application in clinic improved tissue regeneration and restoration of oral and maxillofacial tissue, normalized the diagnosis and treatment, and promoted the development of oral and maxillofacial surgery and translational medicine.