Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (3): 375-378.

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Determination of cyclic adenosine 3′, 5′-monophosphate in fructus jujubae by high performance liquid chromatography

GAO wen, DING Zhao-yi, XU fei, GUO Jian-qiang*   

  1. Centre for Industrial Management and Administration, Capital Medical University, Beijing 100069, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-06-21 Published:2011-06-21
  • Contact: GUO Jian-qiang

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Abstract:

Objective To establish the method of determination of cyclic adenosine 3′, 5′-monophosphate(cAMP) in fructus jujubae by high performance liquid chromatography(HPLC).
Methods HPLC analysis was carried out using MGC18(4.6 mm×250 mm, 5 μm). The mobile phase was 0.05 mol/L KH2PO4-methanol(90：10-10：90), Grade-Washing, the flow rate was 1.0 mL/min, Detected wavelength was set at 254 nm, column temperature was 30 ℃.
Results The linear range for c-AMP was 15.6~500 ng(r=1.000), the average recovery was 100.35%±1.58%, RSD was 1.58%.
Conclusion The method is rapid and accurate with good reproducibility which is used for the quality control of c-AMP in jujubae.

Key words: high performance liquid chromatography, cyclic adenosine 3&prime, 5&prime, monophosphate, fructus jujubae

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