Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (3): 379-383.

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High sensitive method for the detection of aflatoxins in extract from Chinese medicinal herb

ZHAO Shu-rui1, XU Yan-xia2, XU Fei3, GAO Wen3*   

  1. 1. Pharmaceutical Experimental Test Chamber, Medical Experiment and Test Center, Capital Medical University, Beijing 100069,China; 2. Experiment Center, College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China;3. Centre for Industrial Management and Administration, Capital Medical University, Beijing 100069, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-06-21 Published:2011-06-21
  • Contact: GAO Wen

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Abstract:

Objective To establish a rapid method of high performance liquid chromatography(HPLC) plus precolumn derivatization for the determination of aflatoxins(AFT) in Chinese medicine herbal extract.
Methods The Chinese medicinal herbs were extracted with methanol and the extract was cleaned up with a solid phase extraction column. The analytes were eluted on C18 column with different proportion(15%~30%) acetonitrile, water gradient elution.
Results The spiked recoveries were 90%~103.2%. The AFT limits of detection were 0.09 ppb(G1), 0.02 ppb(B2), 0.03 ppb(B1), 0.04 ppb(G2). The results were lower than those of the previous study.
Conclusion The modified method was validated by the analysis of a certified reference material and shown to be useful for the determination of aflatoxins in herbal samples of high acidity.

Key words: high performance liquid chromatography, pre-column derivatization, aflatoxins B1, B2, G1, G2, herbal extracts

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