Effects of protein kinase C-β inhibitor LY333531 on the protein expression of nicotinamide dinucleotide phosphate oxidase and superoxide dismutase in diabetic kidney

doi:10.3969/j.issn.1006-7795.2011.03.017

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (3): 392-396.doi: 10.3969/j.issn.1006-7795.2011.03.017

Previous Articles Next Articles

Effects of protein kinase C-β inhibitor LY333531 on the protein expression of nicotinamide dinucleotide phosphate oxidase and superoxide dismutase in diabetic kidney

GAO Xia¹, YE Feng², ZHOU Chi-yan², LIU Hui-min³, XU Yuan¹, XIA Zheng-yuan^3,4, LEI Shao-qing^3,4*

1. Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China; 2. Department ofBiochemistry and Molecular Biology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China; 3. Departmentof Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China; 4. Department of Pharmacology, School ofBasic Medical Science, Wuhan University, Wuhan 430071, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-06-21 Published:2011-06-21
Contact: LEI Shao-qing

Abstract

Abstract:

Objective To study the effects of protein kinase C(PKC)-β inhibitor LY333531 on nicotinamide dinucleotide phosphate(NADPH) oxidase and superoxide dismutase(SOD) in diabetic kidney.
Methods Twenty-four male SD rats were randomly assigned to control group, diabetic group and diabetic with LY333531〔1 mg/(kg·d)〕 treatment group. After the treatment with LY333531 for four weeks, the levels of blood glucose, kidney weight/body weight, 24-hour urinary albumin, creatinine clearance rate(Ccr), free 15-F2t-Isoprostane, total antioxidant concentration and SOD activity were measured. The subunits P22phox and P67phox of NADPH oxidase, Cu/Zn-SOD and Mn-SOD protein expression were analyzed by Western blotting.
Results The levels of blood glucose, kidney weight/body weight, 24-hour urinary albumin, creatinine clearance rate, 15-F2t-Isoprostane in plasma and kidney, total antioxidant concentrations in plasma, the protein expression of P22phox and P67phox were all increased, but the levels of total antioxidant concentrations in kidney, SOD activity in plasma and kidney, Cu/Zn-SOD and Mn-SOD protein expression were significantly decreased in diabetic group as compared with control group(P<0.05). The treatment with LY333531 significantly prevented all these changes except for blood glucose and MnSOD protein expression.
Conclusion The PKC-β inhibitor LY333531 may protect early diabetic kidney from oxidative injury by inhibiting NADPH oxidase activation and expression, and restoring SOD activity by improving the expression of Cu/Zn-SOD subunit.

Key words: protein kinase C-&beta, inhibitor;LY333531;diabetic nephropathy;nicotinamide dinucleotide phosphate oxidase;superoxide dismutase

CLC Number:

R 587.1

GAO Xia, YE Feng, ZHOU Chi-yan, LIU Hui-min, XU Yuan, XIA Zheng-yuan, LEI Shao-qing, . Effects of protein kinase C-β inhibitor LY333531 on the protein expression of nicotinamide dinucleotide phosphate oxidase and superoxide dismutase in diabetic kidney[J]. Journal of Capital Medical University, 2011, 32(3): 392-396.

0
/ Save 0 / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2011.03.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2011/V32/I3/392

[1]	LI Feng-ting;LI Meng;GENG Jian-guo. Effects of Gubentongluo decoction on tubulointerstitial p38MAPK expression in diabetic nephropathy rats [J]. Journal of Capital Medical University, 2011, 32(6): 816-819.
[2]	JIA Yu-mei;XU Yuan;YANG Jin-kui. Relationship between diabetic retinopathy and diabetic nephropathy [J]. Journal of Capital Medical University, 2011, 32(5): 593-597.
[3]	YANG Guang-ran;A San;FENG Wei;MA Jing;ZHU Xiao-rong;AN Yan-hua;LU Jing-kai;ZHANG Xiu-qing;YANG Jin-kui. Exome sequencing in patients with proliferative diabetic retinopathy [J]. Journal of Capital Medical University, 2011, 32(5): 598-601.
[4]	MA Ya-hong;XIAN Xun-de;YANG Jun-fen;ZHEN Ya-ping;LIU Guo-qing;YANG Jin-kui. Characteristics of HDL2b changes and their significance in prediction of risks for coronary artery disease in type 2 diabetic patients [J]. Journal of Capital Medical University, 2011, 32(5): 609-613.
[5]	REN Hai-rong;HUA Qi. Prognosis of patients with hypertension complicated with glucose metabolic disorder at home and abroad [J]. Journal of Capital Medical University, 2011, 32(5): 634-639.
[6]	HE Yu-lan△, ZHAO Juan△, LIU Xue-mei, ZHANG Fen-yan, LI Juan, YU Hong-wei, WANG Jin-huan, MENG Qing-hua. Clinical significance of GA, FBG, HbA1c in evaluating hepatogenic diabetes carbohydrate metabolic disturbance [J]. Journal of Capital Medical University, 2011, 32(3): 352-355.
[7]	YAN Ying, ZHAO Yong-mei, ZHAO Zhi-wei, WANG Yu-lan, LI Sen, . The Changes in Expression of Endoplasmic Reticulum Stress Related Moleculars GRP78 and CHOP in Hippocampus of Mice with Diabetic Encephalopathy [J]. Journal of Capital Medical University, 2011, 32(1): 90-94.
[8]	WU Yi-ming, CHEN Cheng, ZHANG Jun-qing. Changes in mRNA Expression of Peroxisome Proliferator-activated Receptor-γ Co-activator 1(PGC-1α) in Different Tissues of OLETF Rats [J]. Journal of Capital Medical University, 2010, 31(5): 623-627.
[9]	WANG Rui, JIANG Ping-ping, GONG Chun-xiu, CAO Bing-yan, YAN Gui-jie, LI Yu-chuan, LIU Li. Evaluation of Effect of Four Education Forms for Hospitalized Diabetic Children at Different Age [J]. Journal of Capital Medical University, 2010, 31(2): 197-200.
[10]	ZHANG Gui-ju, SHEN Ying, ZHU Cheng, LIU Min, BAI Guang-hui. Early Diagnosis of Type Ⅰ Diabetic Nephropathy [J]. Journal of Capital Medical University, 2010, 31(1): 123-125.
[11]	SU Zhi-yan, YANG Jin-kui, ZHANG Lin. Relationship between Diabetic Retinopathy and Serum Bilirubin Level [J]. Journal of Capital Medical University, 2010, 31(1): 117-119.
[12]	MENG Xiang-hong, ZHAO Zhi-wei, JI Zhi-juan, ZHANG Jing-yan, WANG Rong. Effect of Analog P165 of APP 5 mer Peptide and Rosiglitazone on Change of Spatial Reference Learning and Memory Ability in Type 3 Diabetes Mellitus(T3DM) Rats [J]. Journal of Capital Medical University, 2010, 31(1): 65-68.
[13]	HONG Xu;MA Qing;CHEN Hai-ping;YU Heng-chi;YIN Jie;HAO Ya-bin. Clinical Characteristics of the Elderly with Metabolic Syndrome [J]. Journal of Capital Medical University, 2009, 30(5): 691-695.
[14]	WANG Li-juan;PAN Huan-feng;GE Huan-qi. The Analysis of Death Causes in 450 Senile Diabetic Inpatients [J]. Journal of Capital Medical University, 2009, 30(4): 471-474.
[15]	XING Jian-ping;YANG Jun-fen;ZHEN Ya-ping;MA Ya-hong;ZHAO Lei;YANG Jin-kui. Prevalence of Impaired Glucose Regulation in Beijing Rural Population and the Necessity to Carrying out Oral Glucose Tolerance Test [J]. Journal of Capital Medical University, 2009, 30(4): 467-470.

Effects of protein kinase C-β inhibitor LY333531 on the protein expression of nicotinamide dinucleotide phosphate oxidase and superoxide dismutase in diabetic kidney

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles 0

Metrics

Comments