Correlation between neurological deficits scores and infarct volume in focal cerebral ischemia in rats

doi:10.3969/j.issn.1006-7795.2013.01.013

Abstract

Abstract:

Objective To investigate the correlation between three scores of neurological deficits and infarct volume of middle cerebral artery occlusion (MCAO) model in rats. Methods A total of 20 Sprague-Dawley (SD) rats were randomly divided into five groups. MCAO models were induced. At five different time points (24 h, 3 d, 7 d, 14 d and 30 d after reperfusion), three grading methods (Longa test, Forelimb Placing test and Ludmila Belayev test) were used to evaluate neurological functions of rats. Then, the volume of infarction was calculated after taking the brain for HE staining. The correlations between neurological function scores and brain infarction volumes were analyzed by ANOVA and correlation.Results From 24 hours to 30 days after reperfusion, the scores of neurological deficits and the volume of infarction were decreased. There were significant differences in the scores of forelimb placing test and Ludmila Belayev test between 7 and 30 days after reperfusing (P<0.05). But the differences of infarction volume at different time points were insignificant (P>0.05). In terms of the correlation between the Ludmila Belayev score and infarction volume, there exists the obvious statistical significance at 3 (P<0.01) and 30 days (P<0.05). Conclusion For the MCAO model of rats, the Ludmila Belayev test can be used to evaluate the extent of injury after cerebral infarction at 3 and 30 days after reperfusion.

Key words: cerebral ischemia, neurological function scoring, infarct volume

CLC Number:

R284.2

WANG Rongliang, LIU Xiaomeng, ZHANG Ying, JI Xunming, LUO Yumin. Correlation between neurological deficits scores and infarct volume in focal cerebral ischemia in rats[J]. Journal of Capital Medical University, 2013, 34(1): 69-74.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.01.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I1/69

References

[1] Wakayama K, Shimamura M, Sata M, et al. Quantitative measurement of neurological deficit after mild (30 min) transient middle cerebral artery occlusion in rats[J]. Brain Res, 2007, 1130(1): 181-187.

[2] Boyko M, Ohayon S, Goldsmith T, et al. Morphological and neuro-behavioral parallels in the rat model of stroke [J]. Behav Brain Res, 2011, 223(1): 17-23.

[3] Longa E Z, Weinstein P R, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.

[4] De Ryck M, Van Reempts J, Borgers M, et al. Photochemical stroke model: flunarizine prevents sensorimotor deficits after neocortical infarcts in rats [J]. Stroke, 1989, 20(10): 1383-1390.

[5] Kim S J, Kim B K, Ko Y J, et al. Functional and histologic changes after repeated transcranial direct current stimulation in rat stroke model [J]. J Korean Med Sci, 2010, 25(10): 1499-1505.

[6] Belayev L, Alonso O F, Busto R, et al. Middle cerebral artery occlusion in the rat by intraluminal suture neurological and pathological evaluation of an improved model [J]. Stroke, 1996, 27(9): 1616-1623.

[7] Fisher M, Feuerstein G, Howells D W, et al. Update of the stroke therapy academic industry roundtable preclinical recommendations [J]. Stroke, 2009, 40(6): 2244-2250.

[8] Spilker J, Kong G, Barch C, et al. Using the NIH Stroke Scale to assess stroke patients. The NINDS rt-PA Stroke Study Group [J]. J Neurosci Nurs, 1997, 29(6):384-392.

[9] Criddle L M, Bonnono C, Fisher S K. Standardizing stroke assessment using the National Institutes of Health Stroke Scale [J]. J Emerg Nurs, 2003, 29(6):541-546.

[10] Fisher M. Recommendations for standard regarding preclinical neuroprotective and restorative drug development [J]. Stroke, 1999, 30(12): 2752-2758.

[11] 刘小蒙,王荣亮,罗玉敏.大鼠脑缺血神经功能缺损行为学评价的研究进展[J].中国脑血管病杂志,2012,9(4):208-212.

[12] Wahl F, Alix M, Plotkine M, et al. Neurological and behavioral outcomes of focal cerebral ischemia in rats [J]. Stroke, 1992, 23(2): 267-272.

[13] Chauveau F, Cho T H, Riou A, et al. Does acute behavioral testing reflect successful ischemia in rats with transient middle cerebral artery occlusion? [J]. Int J Stroke, 2012, 7(6): 465-472.

[14] Wegener S, Weber R, Ramos-Cabrer P, et al. Subcortical lesions after transient thread occlusion in the rat: T2-weighted magnetic resonance imaging findings without corresponding sensorimotor deficits [J]. J Magn Reson Imaging, 2005, 21(4): 340-346.

[15] van der Zijden J P, Bouts M J, Wu O, et al. Manganese-enhanced MRI of brain plasticity in relation to functional recovery after experimental stroke [J]. J Cereb Blood Flow Metab, 2008, 28(4): 832-840.

[16] Henninger N, Bouley J, Bratane BT, et al. Laser Doppler flowmetry predicts occlusion but not tPA-mediated reperfusion success after rat embolic stroke [J]. Exp Neurol, 2009, 215(2): 290-297.

[17] Ashioti M, Beech J S, Lowe A S, et al. Multi-model characterisation of the neocortical clip model of focal cerebral ischaemia by MRI, behaviour and immunohistochemistry [J]. Brain Res, 2007, 1145:177-189.

[18] 闫峰,吉训明,宣芸,等.新西兰家兔大脑中动脉闭塞脑缺血模型建立的经验[J].首都医科大学学报,2010,31(1):79-83.

[19] Walberer M, Stolz E, Muller C, et al. Experimental stroke: ischaemic lesion volume and oedema formation differ among rat strains (a comparison between wistar and Sprague-Dawley rats using MRI)[J]. Lab Anim, 2006, 40(1): 1-8.

[20] Encarnacion A, Horie N, Keren-Gill H, et al. Long-term behavioral assessment of function in an experimental model for ischemic stroke [J]. J Neurosci Methods, 2011, 196(2): 247-257.

[21] Zansinger S, Hungerhuber E, Baethmann A, et al. Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms [J] Brain Res, 2000, 863(1-2): 94-105.

[22] He Z, Yamawaki T, Yang S, et al. Experimental model of small deep infarcts involving the hypothalamus in rats, Changes in body temperature and postural reflex [J]. Stroke, 1999, 30(12): 2743-2751.

[23] Kuroiwa T, Okeda R. Checkpoints and pitfalls in the experimental neuropathology of circulatory disturbance [J]. Neuropathology, 2003, 23(1):79-89.

[24] Hackam DG, Redelmeier DA. Translation of research evidence from animals to humans [J]. JAMA, 2006, 296(14): 1731-1732.

[25] Jung SH, Kim YK, Kim SE, et al. Prediction of Motor Function Recovery after Subcortical Stroke: Case Series of Activation PET and TMS Studies [J]. Ann Rehabil Med, 2012, 36(4):501-511.