Adoptive transfer of murine bone-marrow derived macrophages tracing in vivo and application in hypertensive cardiac injury

doi:10.3969/j.issn.1006-7795.2013.03.014

Abstract

Abstract:

Objective Two types of mice enhanced green fluorescent protein (EGFP) transgenic mice and CD45 type I (CD45.1) mice) to observe whether the adoptive transfusion of murine bone-marrow derived macrophages(BMM)might affect the hypertensive damage to heart in murine hypertension model. Methods After obtaining bone marrow cells from EGFP transgenic mice and inducing cells with macrophage colony-stimulating factor (M-CSF) to BMM, wild-type mice received injection of 2×10⁶ EGFP⁺BMMs or phosphate-buffered saline (PBS) through tail vein and observed survival proportion of EGFP⁺cells in peripheral blood at 1st day and 7th day. Then we collected heart, liver and spleen to determine expression of GFP⁺ cells. Likewise we used CD45.1 BMM to inject into CD45.2 mice and determine the proportion of CD45.1⁺ cells in peripheral blood. Then angiotensin Ⅱ (Ang Ⅱ) was infused to the mice and EGFP⁺BMMs or PBS was infused as previously described. After 7 days infiltration of EGFP⁺BMM, inflammation and fibrosis in heart were determined.Results The proportion of EGFP⁺cells in peripheral blood in EGFP⁺BMMs injection group were 1st day:(4.6±0.5)%; 7th day:(4.4±0.5)% while none in control group. After 7 days GFP⁺cells expressed in spleen while none in liver, kidney or heart. The proportion of CD45.1⁺cells in peripheral blood in CD45.1⁺BMMs injection group was (4.3±0.4)% while none in control group. In contrast to control group, AngⅡ infused group had accelerated increased blood pressure, infiltration of inflammatory cells and collagen deposition. Injection of EGFP⁺BMMs could infiltrate in heart and accelerate inflammation and fibrosis, but did not affect blood pressure. Conclusion This study proved that adoptive transfuse BMMs could survive in recipient and accelerate cardiac fibrosis. We settled the problem of tracing the adoptive transfused BMM in vivo.

Key words: bone-marrow derived macrophages, adoptive transfer, tracing technique, hypertension

CLC Number:

R 541.3

LI Yulin, WU Yina, ZHANG Congcong, KAN Xiaoyu, A Xi, ZHAO Wei, WANG Lvya, DU Jie. Adoptive transfer of murine bone-marrow derived macrophages tracing in vivo and application in hypertensive cardiac injury[J]. Journal of Capital Medical University, 2013, 34(3): 391-397.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.03.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I3/391

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