Abstract:

Objective To determine the safety of acer truncatum bunge leaves extract (AT). Methods Acute oral toxicity testing of AT was performed in mice. Genotoxicity of AT was assessed by bone marrow micronucleus, mice sperm abnormality and bacterial reverse mutation tests. The subchronic toxicity study of AT was used via ninety days feeding test. Results The oral maximum tolerated dose of AT was more than 10g/kg, which belonged to an actual non-toxicity substance. The results of genotoxicity profiles were all negative. There was no significant difference in toxicity between control and AT treated animals with respect to general condition, body weight, food efficiency, food consumption and clinical chemistry parameters throughout the treatment at dose 1-2g/kg. But compared with the control group, body weight and food consumption were significantly lower in 6g/kg group male rats. And compared with the control group, hypoglycemic effect was indicated in 6g/kg group male rats after 45 days treatment. Conclusion The AT is safe and shows no evidence of observed adverse effect up to the 2g/kg dose.

Key words: acer truncatum bunge leaves extract, acute toxicity, genotoxicity, ninety days feeding study