Expressions of p-JAK1 and BDNF in experimental autoimmune encephalomyelitis in mice induced by myelin oligodendrocyte glycoprotein at different doses

doi:10.3969/j.issn.1006-7795.2014.06.010

Abstract

Abstract:

Objective To compare the expressions of phospho-Janus kinase 1(p-JAK1)and brain-derived neurotrophic factor(BDNF) of experimental autoimmune encephalomyelitis(EAE) in C57BL/6 mice models induced by myelin oligodendrocyte glycoprotein 35-55(MOG_35-55) at different dosages. Methods Thirty female SPF-grade C57BL/6 mice with 18-22 gram body weight were divided randomly into three groups: control group and EAE model groups(MOG_35-55 50 μg dosage group and MOG_35-55 200 μg dosage group). The mice of the two model groups were injected subcutaneously over flanks with the antigen containing 50 μg, 200 μg MOG_35-55/mouse and complete Freund's andjuvant(CFA) in the same volume, respectively. The mice of the control group were injected in the same way phosphate buffered saline(PBS) without containing MOG_35-55; 500 ng pertussis toxin(PTX) in 0.2 mL phosphate buffer solution(PBS) was given by intraperitoneal injection to the mice of the two model groups at 0 and 48 h post-immunization. The mice in control group were injected with PBS in the same way. The disease incidence, death rate, body weight and neurological score of the mice were observed. Meanwhile, the expression of JAK1 and p-JAK1 in cortex were examined by western blotting and brain-derived neurotrophic factor(BDNF) evaluated by immunohistochemical staining. Results The C57BL/6 mouse model of EAE was successfully induced by two different dosages of MOG_35-55. The expression of p-JAK1 in cortex were increased while BDNF decreased. However, the influence of MOG_35-55 200 μg dosage group on loss of weight, neurological score and the expression of p-JAK1 seemed to be more significant than MOG_35-55 50 μg dosage group. Conclusion The mouse model of immune-induced EAE was successfully established with MOG_35-55 200 μg and this EAE mouse model is stable and can be used in the drug research of multiple sclerosis(MS).

Key words: experimental autoimmune encephalomyelitis, myelin oligodendrocyte glycoprotein, phospho-Janus kinase 1, brain-derived neurotrophic factor

CLC Number:

R967

Qu Zhao, Zhang Li, Li Lin, Wang Qi, Yin Linlin. Expressions of p-JAK1 and BDNF in experimental autoimmune encephalomyelitis in mice induced by myelin oligodendrocyte glycoprotein at different doses[J]. Journal of Capital Medical University, 2014, 35(6): 725-729.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.06.010

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I6/725

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