Effects of sevoflurane on MAC and expression of protein PARP-1 in different encephalic regions of neonatal rats

doi:10.3969/j.issn.1006-7795.2015.04.024

Abstract

Abstract:

Objective To measure the sevoflurane minimum alveolar concentration(MAC) and to investigate the effect of sevoflurane anesthesia on the expression of poly(ADP-ribose)polymerase-1(PARP-1) protein in various brain regions in 7-day postnatal mice. Methods One hundred neonatal Sprague-Dawley(SD) rats(7 days postnatal, P7) were selected in this study. The sevoflurane MAC was determined in littermates(aged 7 days, n=10) using tail-clamp stimulation in a bracketing study design. At the end of anesthesia with different concentration gradient and duration gradient of sevoflurane, arterial blood samples from left ventricular of 3 random rats of each anesthesia maintenance were obtained immediately for blood gas analysis, and choose the maximum concentration and duration to keep the normal respiratory parameters. Then the neonatal mice were randomly divided into three different groups: the control group(A), the sham anesthesia group(B), and the anesthesia group(C), the expression contents of PARP-1 protein in different brain regions were tested by Western blotting 6 hours after exposure. Results The sevoflurane MAC of the 7 days mice was(2.64±0.12)%, 0.8MAC and 4 h anesthesia was set as the most suitable concentration rate and the anesthesia duration, respectively. Compared to the group A(100%), the PARP-1 expression in the hippocampus(270±22)%, the cortex of the temporal lobe(160±15)%, and the cortex of the frontal lobe(165±8)% of the group C was significantly increased(P<0.05), however, the PARP-1 expression in the thalamus(115±26)% was not statistically significant between the Goup A and C. The parameters of the Group B was not significantly different with Group A(P>0.05). Conclusion The sevoflurane MAC of the 7 days mice was 2.64%. 0.8 MAC sevoflurane anesthesia for 4 h may induce neuron apoptosis in cortex and hippocampus of the 7-days mice brain, and especially in hippocampus area.

Key words: sevoflurane, newborn rats, minimum alveolar concentration, poly(ADP-ribose) polymerase-1

CLC Number:

R595.5

Xie Sining, Ye Hong, An Lixin, Li Junfa. Effects of sevoflurane on MAC and expression of protein PARP-1 in different encephalic regions of neonatal rats[J]. Journal of Capital Medical University, 2015, 36(4): 634-639.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.04.024

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I4/634

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