Abstract:

Objective To investigate the feasibility for fabricating the 3-dimensional scaffolds with collagen-chitosan applied to tissue engineering of blood vessel. Methods Different proportion of collagen to chitosan suspensions were used as the biomaterials to construct 3-Dscaffolds in vitro. And the 3-Dstructure in the scaffolds were observed. The scaffolds biodegradability and biocompatible property were observed by imbedding them subcutaneously or dipping in the solution of collagenase (Ⅰ), bacteriolysin, trypsinase solutions. Results The fabricated scaffolds had the relatively uniform structure, with perfect biocompatibility. The different proportions for collagen to chitosan could work on the construction character in scaffolds, the chitosan could influence collagenase degradation for scaffolds and prolong the degradation progress in vivo. Conclusion The 3-dimentional scaffolds made from collagen-chitosan, with relatively uniform and excellent biocompatibility, can be used in vascular tissue engineering research.

Key words: tissue engineering, collagen, chitosan, three-dimensional scaffolds, cell biocompatibility