Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (5): 628-632.doi: 10.3969/j.issn.1006-7795.2010.05.022

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Separation and Purification of Total Polyphenols from Acer truncatum Bunge Leaves with Macroporous Adsorption Resin

YE Yan-bin1, LI Xi-xuan2, GAO Wen3, FAN Yuan-jie1, YANG Hua1, ZHANG Feng2*, ZHANG Ying-xia2   

  1. 1. Experimental Centre, College of Chemical Biology and Pharmaceutical Sciences, Capital Medical University; 2. Department of Chemical Biology, College of Chemical Biology and Pharmaceutical Sciences, Capital Medical University; 3. Food and Drug Safety Evaluation Center, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-21 Published:2010-10-21
  • Contact: ZHANG Feng

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Abstract:

Objective To study the optimal techniques for separation of total polyphenols from the leaves of Acer truncatum bunge with macroporous adsorption resins.Methods The static and dynamic adsorption-desorption characteristics were taken as index to select the macroporous adsorption resin. The separating efficiency was evaluated by measuring the concentration of total polyphenols in A. truncatum. with UV spectrophotometer.Results The HPD100 macroporous adsorption resin had the best separating efficiency. The optimum separation conditions of macroporous adsorption resin HPD100 are as follows: the concentration of the extract from A. truncatum. leaves solution is 5 mg/mL, and the velocity is 2 mL/min, with 4BV of 70% ethanol to elute. Conclusion The total polyphenols in the leaves of A. truncatum was able to be easily separated and purified by HPD100 resin, and its adsorption-desorption characteristics were stable under the optimum conditions mentioned above. The original content of polyphenols was 28.55%, after purification with HPD100, it could reach 39.52%.

Key words: Acer truncatum bunge, polyphenols, macroporous adsorption resins

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